用于测定血清胸腺生成素的改良放射免疫分析技术。

Improved radioimmunoassay technique for measuring serum thymopoietin.

作者信息

Lisi P J, Teipel J W, Goldstein G, Schiffman M

出版信息

Clin Chim Acta. 1980 Oct 23;107(1-2):111-9. doi: 10.1016/0009-8981(80)90420-9.

Abstract

An improved radioimmunoassay technique for measuring serum thymopoietin has been developed which obviates the spurious displacements previously obtained with serum samples, and increases sensitivity to 20 pg. Key improvements involved further purification of labeled thymopoietin, sequential incubations to enhance sensitivity and the use of charcoal-treated serum blanks to render controls and unknown samples comparable to the standard curve.

摘要

已开发出一种改进的放射免疫测定技术来测量血清胸腺生成素，该技术消除了先前血清样本出现的假置换现象，并将灵敏度提高到20皮克。关键改进包括进一步纯化标记的胸腺生成素、采用顺序孵育以提高灵敏度，以及使用经活性炭处理的血清空白对照，使对照品和未知样品与标准曲线具有可比性。