大鼠α-乳白蛋白启动子的保守区域是体外蛋白质结合的靶位点。
Conserved region of the rat alpha-lactalbumin promoter is a target site for protein binding in vitro.
作者信息
Lubon H, Hennighausen L
机构信息
Laboratory of Biochemistry and Metabolism, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892.
出版信息
Biochem J. 1988 Dec 1;256(2):391-6. doi: 10.1042/bj2560391.
Nuclear extracts from mammary glands of lactating rats contain DNA binding protein(s) with high affinity to the region -125 to -85 of the rat alpha-lactalbumin promoter. The protected sequence is part of a mammary conserved sequence described by Hall et al. [(1987) Biochem. J. 242, 735-742]. Comparison of the DNA protein-binding sites in the 5' flanking sequence of the rat alpha-lactalbumin and mouse whey acidic protein genes suggests that the consensus sequence 5'-TGGCARNNWCKKC-3' is the protein(s) recognition site. This binding site is part of a highly conserved region [consensus sequence, TGGCAGSCTCGGCST(G)YTCTCTCT(NTG)TGGCARA] present in the promoter of four whey protein genes.
来自泌乳大鼠乳腺的核提取物含有与大鼠α-乳白蛋白启动子-125至-85区域具有高亲和力的DNA结合蛋白。受保护的序列是Hall等人描述的乳腺保守序列的一部分。[(1987) Biochem. J. 242, 735 - 742]。大鼠α-乳白蛋白和小鼠乳清酸性蛋白基因5'侧翼序列中DNA蛋白结合位点的比较表明，共有序列5'-TGGCARNNWCKKC-3'是该蛋白的识别位点。该结合位点是四个乳清蛋白基因启动子中存在的高度保守区域[共有序列，TGGCAGSCTCGGCST(G)YTCTCTCT(NTG)TGGCARA]的一部分。
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