Franco E L, Walls K W, Sulzer A J, Soto J C
J Immunoassay. 1983;4(4):373-93. doi: 10.1080/15321818308057016.
The reversed enzyme-labelled antigen immunoassay (R-EIA), based on the capture of serum immunoglobulin M antibodies (IgM) and subsequent addition of Toxoplasma gondii soluble antigen tagged with peroxidase and substrate, was evaluated comparatively with the IgM-indirect immunofluorescence test (IgM-IIF) for the detection of anti-toxoplasma IgM antibodies in sera from individuals with diagnosed acute acquired toxoplasmosis. Additional serum groups from normal healthy individuals and sera presenting possible nonspecific reactivities were also evaluated. Complete specificity of R-EIA was shown. There was no correlation between the magnitude of R-EIA results and IgM-IIF titers, but a positive (although not linear) correlation was found between R-EIA and the IgM-IIF titers obtained after adsorption of sera with Staphylococcus aureus protein A. Direct labelling of the antigen by a simple coupling technique facilitated the assay standardization and improved its signal-to-noise ratio.
基于捕获血清免疫球蛋白M抗体（IgM），随后添加用过氧化物酶和底物标记的弓形虫可溶性抗原的反向酶标记抗原免疫测定法（R-EIA），与IgM间接免疫荧光试验（IgM-IIF）进行了比较，用于检测确诊为急性获得性弓形虫病个体血清中的抗弓形虫IgM抗体。还评估了来自正常健康个体的其他血清组以及呈现可能非特异性反应性的血清。结果显示R-EIA具有完全特异性。R-EIA结果的大小与IgM-IIF滴度之间没有相关性，但在用金黄色葡萄球菌A蛋白吸附血清后获得的R-EIA与IgM-IIF滴度之间发现了正相关（尽管不是线性的）。通过简单的偶联技术直接标记抗原有助于测定标准化并提高其信噪比。
