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酿酒酵母GAL4基因编码的两种蛋白质的鉴定

Identification of two proteins encoded by the Saccharomyces cerevisiae GAL4 gene.

作者信息

Laughon A, Driscoll R, Wills N, Gesteland R F

出版信息

Mol Cell Biol. 1984 Feb;4(2):268-75. doi: 10.1128/mcb.4.2.268-275.1984.

DOI:10.1128/mcb.4.2.268-275.1984
PMID:6366517
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC368691/
Abstract

We placed the Saccharomyces cerevisiae GAL4 gene under control of the galactose regulatory system by fusing it to the S. cerevisiae GAL1 promoter. After induction with galactose, GAL4 is now transcribed at about 1,000-fold higher levels than in wild-type S. cerevisiae. This regulated high-level expression has enabled us to tentatively identify two GAL4-encoded proteins.

摘要

我们通过将酿酒酵母GAL4基因与酿酒酵母GAL1启动子融合，使其置于半乳糖调节系统的控制之下。用半乳糖诱导后，GAL4的转录水平现在比野生型酿酒酵母高约1000倍。这种受调控的高水平表达使我们能够初步鉴定出两种由GAL4编码的蛋白质。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e49/368691/fe1a012cd3ba/molcellb00144-0061-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e49/368691/fb6a9a581633/molcellb00144-0059-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e49/368691/5199468a7c69/molcellb00144-0060-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e49/368691/f79d72c787d4/molcellb00144-0061-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e49/368691/fe1a012cd3ba/molcellb00144-0061-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e49/368691/fb6a9a581633/molcellb00144-0059-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e49/368691/5199468a7c69/molcellb00144-0060-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e49/368691/f79d72c787d4/molcellb00144-0061-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e49/368691/fe1a012cd3ba/molcellb00144-0061-b.jpg

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本文引用的文献

1
ENZYMATIC EXPRESSION AND GENETIC LINKAGE OF GENES CONTROLLING GALACTOSE UTILIZATION IN SACCHAROMYCES.酿酒酵母中控制半乳糖利用的基因的酶表达与遗传连锁
Genetics. 1964 May;49(5):837-44. doi: 10.1093/genetics/49.5.837.
2
Deletion analysis of the Saccharomyces GAL gene cluster. Transcription from three promoters.酿酒酵母GAL基因簇的缺失分析。来自三个启动子的转录。
J Mol Biol. 1981 Oct 25;152(2):317-34. doi: 10.1016/0022-2836(81)90245-x.
3
Physical analysis of mating-type loci in Saccharomyces cerevisiae.酿酒酵母交配型位点的物理分析。
通过GAL11P募集转录机制：GAL4二聚化结构域的结构与相互作用
Genes Dev. 2001 Apr 15;15(8):1007-20. doi: 10.1101/gad.873901.
4
Molecular Dissection of the 5' Region of no-on-transientA of Drosophila melanogaster Reveals cis-Regulation by Adjacent dGpi1 Sequences.黑腹果蝇无瞬时A蛋白5'区域的分子剖析揭示了相邻dGpi1序列的顺式调控作用。
Genetics. 2001 Feb;157(2):765-75. doi: 10.1093/genetics/157.2.765.
5
Affecting gene expression by altering the length and sequence of the 5' leader.通过改变5'前导序列的长度和序列来影响基因表达。
Proc Natl Acad Sci U S A. 1984 Dec;81(24):7698-702. doi: 10.1073/pnas.81.24.7698.
6
Amino terminus of the yeast GAL4 gene product is sufficient for nuclear localization.酵母GAL4基因产物的氨基末端足以实现核定位。
Proc Natl Acad Sci U S A. 1984 Oct;81(19):5951-5. doi: 10.1073/pnas.81.19.5951.
7
Specific protein binding to far upstream activating sequences in polymerase II promoters.特异性蛋白质与聚合酶II启动子中远上游激活序列的结合。
Proc Natl Acad Sci U S A. 1985 Jan;82(1):43-7. doi: 10.1073/pnas.82.1.43.
8
Reovirus hemagglutinin mRNA codes for two polypeptides in overlapping reading frames.呼肠孤病毒血凝素mRNA在重叠阅读框中编码两种多肽。
Proc Natl Acad Sci U S A. 1985 Jan;82(1):48-52. doi: 10.1073/pnas.82.1.48.
9
Mutations that inactivate a yeast transcriptional regulatory protein cluster in an evolutionarily conserved DNA binding domain.在一个进化上保守的DNA结合结构域中使酵母转录调节蛋白簇失活的突变。
Proc Natl Acad Sci U S A. 1987 Apr;84(8):2401-5. doi: 10.1073/pnas.84.8.2401.
10
Interaction of GAL4 and GAL80 gene regulatory proteins in vitro.体外GAL4和GAL80基因调控蛋白的相互作用
Mol Cell Biol. 1987 Oct;7(10):3446-51. doi: 10.1128/mcb.7.10.3446-3451.1987.
Cold Spring Harb Symp Quant Biol. 1981;45 Pt 2:961-81. doi: 10.1101/sqb.1981.045.01.113.
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Proc Natl Acad Sci U S A. 1982 Nov;79(22):6971-5. doi: 10.1073/pnas.79.22.6971.
10
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Proc Natl Acad Sci U S A. 1982 Nov;79(22):6827-31. doi: 10.1073/pnas.79.22.6827.