酿酒酵母GAL4基因编码的两种蛋白质的鉴定
Identification of two proteins encoded by the Saccharomyces cerevisiae GAL4 gene.
作者信息
Laughon A, Driscoll R, Wills N, Gesteland R F
出版信息
Mol Cell Biol. 1984 Feb;4(2):268-75. doi: 10.1128/mcb.4.2.268-275.1984.
Abstract
We placed the Saccharomyces cerevisiae GAL4 gene under control of the galactose regulatory system by fusing it to the S. cerevisiae GAL1 promoter. After induction with galactose, GAL4 is now transcribed at about 1,000-fold higher levels than in wild-type S. cerevisiae. This regulated high-level expression has enabled us to tentatively identify two GAL4-encoded proteins.
摘要
我们通过将酿酒酵母GAL4基因与酿酒酵母GAL1启动子融合，使其置于半乳糖调节系统的控制之下。用半乳糖诱导后，GAL4的转录水平现在比野生型酿酒酵母高约1000倍。这种受调控的高水平表达使我们能够初步鉴定出两种由GAL4编码的蛋白质。
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