Matsubara T, Spycher M A, Rüttner J R, Fehr K
Rheumatol Int. 1983;3(4):153-9. doi: 10.1007/BF00541594.
The distribution of fibronectin in rheumatoid arthritis (RA) synovium has been investigated by light and electron microscopic immunohistochemistry using an antihuman fibronectin antibody. Heavy accumulation of fibronectin was observed in the lining layer and the areas of proliferation of fibroblasts. Rough endoplasmic reticulum (RER) and peripheral vesicles of proliferated type B lining cells and fibroblasts contained large amounts of fibronectin. Thus these cells seem to participate actively in the local synthesis and secretion of this glycoprotein. Type A lining cells and migrated mononuclear phagocytes contained many phagolysosomes in some of which dense accumulation of fibronectin was observed. Some of the materials in the phagolysosomes, with dense accumulation of fibronectin, resembled the fibrinous material-fibronectin complexes frequently seen in the pericellular spaces. Accordingly fibronectin seems to play a role in the clearance of fibrinous materials by these phagocytes. The proliferated capillaries and small vessels possessed multilamellated basement membranes with heavy accumulation of fibronectin. However, RER or Golgi apparatus of the endothelial cells contained no detectable amounts of fibronectin. This indicates that these cells do not actively participate in the synthesis of fibronectin and that the majority of this glycoprotein in the basement membranes originates in fibronectin from blood vessel exudate. Fibrinous material-fibronectin complexes were frequently seen on the endothelial cell surfaces. Circulating platelets and mononuclear cells occasionally came in contact with these complexes, suggesting an association of fibronectin with the formation and clearance of thrombi in the vascular lumina at the inflammatory sites of RA synovium.
利用抗人纤连蛋白抗体，通过光镜和电镜免疫组织化学方法研究了类风湿性关节炎（RA）滑膜中纤连蛋白的分布。在衬里层和成纤维细胞增殖区域观察到纤连蛋白大量积聚。增殖的B型衬里细胞和成纤维细胞的粗面内质网（RER）和周边囊泡含有大量纤连蛋白。因此，这些细胞似乎积极参与了这种糖蛋白的局部合成和分泌。A型衬里细胞和迁移的单核吞噬细胞含有许多吞噬溶酶体，其中一些观察到纤连蛋白密集积聚。吞噬溶酶体中的一些物质，伴有纤连蛋白的密集积聚，类似于在细胞周间隙中常见的纤维状物质 - 纤连蛋白复合物。因此，纤连蛋白似乎在这些吞噬细胞清除纤维状物质中起作用。增殖的毛细血管和小血管具有多层基底膜，纤连蛋白大量积聚。然而，内皮细胞的RER或高尔基体中未检测到纤连蛋白。这表明这些细胞不积极参与纤连蛋白的合成，并且基底膜中的大部分这种糖蛋白起源于来自血管渗出液的纤连蛋白。在内皮细胞表面经常可见纤维状物质 - 纤连蛋白复合物。循环中的血小板和单核细胞偶尔会与这些复合物接触，提示纤连蛋白与RA滑膜炎症部位血管腔内血栓的形成和清除有关。
