Maruyama H, Mori T, Shimano T, Inaji H, Chu T M, Kosaki G
Ann N Y Acad Sci. 1983;417:240-50. doi: 10.1111/j.1749-6632.1983.tb32867.x.
PCAA, a glycoprotein antigen fractionated from the ascites fluid of a patient with pancreatic cancer and sharing an identical immunogenicity with Gelder's POA, and PaA, a novel pancreatic tissue antigen, were studied immunohistologically. Serial paraffin sections were prepared from surgical specimens of 11 cases of pancreatic cancer, 15 of gastric cancer, 12 of colonic cancer, and 2 of gallbladder cancer; these were then subjected to immunofluorescence and immunoperoxidase stainings. In noncancerous tissues, PCAA was detected unexpectedly at goblet cells of the whole intestine, but was completely absent in pancreatic tissues, while PaA was not demonstrated in intestinal tissues, but was positive at acinar cells of the pancreas. In pancreatic cancer tissues, PCAA and PaA were detected at apical cytoplasm of cancer cells, although positive cells were in different proportions and had different distributions. PCAA-positive cells were mucin-producing (positive in PAS-alcian blue staining) and were well differentiated histologically, while PaA-positive cells were less differentiated and poor in mucin production. Among 11 cases of pancreatic cancer, both PCAA- and PaA-positive cells were demonstrated in 3 cases, PCAA-positive cells alone were found in 3 cases, and PaA-positive cells alone were seen in 4 cases. In 27 gastrointestinal cancer tissues, PCAA was detected in 7 cases of mucin-producing cancer, and PaA was demonstrated in 2 cases of poorly differentiated adenocarcinoma.
对从一名胰腺癌患者腹水中分离出的与盖尔德氏POA具有相同免疫原性的糖蛋白抗原PCAA以及一种新型胰腺组织抗原PaA进行了免疫组织学研究。从11例胰腺癌、15例胃癌、12例结肠癌和2例胆囊癌的手术标本中制备连续石蜡切片，然后进行免疫荧光和免疫过氧化物酶染色。在非癌组织中，意外地在整个肠道的杯状细胞中检测到PCAA，但在胰腺组织中完全不存在，而PaA在肠道组织中未显示，但在胰腺腺泡细胞中呈阳性。在胰腺癌组织中，尽管阳性细胞比例不同且分布不同，但在癌细胞的顶端细胞质中检测到PCAA和PaA。PCAA阳性细胞产生黏液（PAS-阿尔辛蓝染色呈阳性），组织学上分化良好，而PaA阳性细胞分化较差且黏液产生较少。在11例胰腺癌中，3例同时显示PCAA和PaA阳性细胞，3例仅发现PCAA阳性细胞，4例仅见PaA阳性细胞。在27例胃肠道癌组织中，7例黏液产生性癌检测到PCAA，2例低分化腺癌显示PaA。
