Bondarenko V M, Marakusha B I
Antibiotiki. 1984 Feb;29(2):124-6.
Mutations resulting in acquisition of the resistance to rifampicin, streptomycin, neamine, polymyxin M, mecillinam and high concentrations of nalidixic acid were studied with respect to their effect on virulence of Shigella flexneri by transduction of the markers from the spontaneous avirulent mutants to the initial strain. It was shown that the acquisition of the determinants of the resistance to rifampicin, streptomycin, neamine (nea A) and high concentration of nalidixic acid resulted in 100 per cent of the cases in losing the transductant capacity for causing keratoconjunctivitis in guinea-pigs. With the mutations resulting in the acquisition of resistance to neamine (nea B) and mecillinam virulence was lost only in a part of the clones (33 and 53 per cent respectively). The changes in the pathogenic properties of the transductants with the acquired markers of the resistance to the above antibiotics were associated with impairment of the bacterial cell vital structures. It is suggested that the genetically characterized avirulen immunogenetic mutants be used as potential vaccinal strains.
通过将自发无毒突变体的标记物转导至初始菌株，研究了导致对利福平、链霉素、新霉素、多粘菌素M、美西林和高浓度萘啶酸产生抗性的突变对福氏志贺氏菌毒力的影响。结果表明，获得对利福平、链霉素、新霉素（nea A）和高浓度萘啶酸的抗性决定簇后，100%的转导子丧失了在豚鼠中引起角结膜炎的能力。对于导致对新霉素（nea B）和美西林产生抗性的突变，仅部分克隆（分别为33%和53%）丧失了毒力。具有上述抗生素抗性获得性标记的转导子致病特性的变化与细菌细胞重要结构的损伤有关。建议将经基因鉴定的无毒免疫遗传突变体用作潜在的疫苗菌株。
