Anisimova L A, Zakharieva S, Boronin A M
Antibiotiki. 1982 Oct;27(10):766-70.
The clinical strain BS205 of P. aeruginosa is characterized by a high level of resistance to streptomycin, kanamycin, chloramphenicol, sulfanilamide and mercuric chloride. These markers can be transferred to P. aeruginosa PAO by means of transduction with phage F116L or mobilization with plasmid RP4. In the same way as in the initial strain of P. aeruginosa BS205 no plasmid DNA is detected in transducers or transconjugants. After transference to the strains of the transducers or transconjugants containing markers Sm, Km, Cm, Su, and Hg. plasmid Rip 64 of the incompatibility group is eliminated from the cells of these strains when they are grown on the nonselective medium. The genes of resistance to streptomycin, kanamycin, chloramphenicol, sulfanilamide and mercury and the gene(s) of incompatibility specific of the plasmid of the incompatibility group P-3 are included in the DNA fragment of the size of about 24 megadalton. This fragment is probably a defective plasmid not capable of autonomic existence which is integrated into the bacterial chromosome of P. aeruginosa BS205.
铜绿假单胞菌临床菌株 BS205 的特征是对链霉素、卡那霉素、氯霉素、磺胺和氯化汞具有高度抗性。这些标记可通过噬菌体 F116L 转导或质粒 RP4 转移作用转移至铜绿假单胞菌 PAO。与铜绿假单胞菌初始菌株 BS205 一样，在转导子或接合子中未检测到质粒 DNA。在将含有链霉素、卡那霉素、氯霉素、磺胺和汞抗性标记转移至转导子或接合子菌株后，当这些菌株在非选择性培养基上生长时，不相容群的质粒 Rip 64 会从这些菌株的细胞中消除。对链霉素、卡那霉素、氯霉素、磺胺和汞的抗性基因以及不相容群 P - 3 质粒特异性的不相容基因包含在大小约为 24 兆道尔顿的 DNA 片段中。该片段可能是一个不能自主存在的缺陷质粒，它整合到了铜绿假单胞菌 BS205 的细菌染色体中。
