Germani Y, Dassy B, Le Gonidec G, Guesdon J L
Ann Immunol (Paris). 1984 Mar-Apr;135C(2):251-60.
The titration of rabbit anti-Escherichia coli heat-labile enterotoxin antibodies by an immunosorbent assay (ELISA) generally uses a GM1 coating step to lend specificity to the ELISA reaction at a time when the method is tempered by a lack of purified E. coli toxin. We have developed an adsorption method of the toxin to the polystyrene in order to simplify the technique. Three different immunosorbent preparations were tested to determine which of them yielded the most sensitive results.
通过免疫吸附测定法（ELISA）滴定兔抗大肠杆菌热不稳定肠毒素抗体时，由于缺乏纯化的大肠杆菌毒素，该方法受到限制，此时通常采用GM1包被步骤来增强ELISA反应的特异性。我们开发了一种毒素吸附到聚苯乙烯上的方法，以简化该技术。测试了三种不同的免疫吸附制剂，以确定哪种制剂产生的结果最灵敏。
