来自酿酒酵母的异亮氨酸tRNA IAU的序列。

Sequence of tRNA Ile IAU from brewer's yeast.

作者信息

Pixa G, Dirheimer G, Keith G

出版信息

Biochem Biophys Res Commun. 1984 Mar 30;119(3):905-12. doi: 10.1016/0006-291x(84)90859-3.

Abstract

The nucleotide sequence of tRNAIle from brewer's yeast Saccharomyces cerevisiae was determined. Its primary structure is pG-G-U-C-U-C-U-U-m1G-m2G- C-C-C-A-G-D-D-G-G-D-D-A-A-G-G-C-A-C-C-G-U-G-C-U-I-A-U-t6 A-A-C-G-C-G-G-G-GA-D-m5 C-A-G-C-G-G-T-psi-C-G-m1 A-U-C-C-C-G-C-U-A-G-A-G-A-C-C-A-C-C-A. Its anticodon is I-A-U. It should therefore recognize the three isoleucine codons and is for this reason probably the only isoacceptor tRNA for isoleucine in brewer's yeast. It presents a large homology with its counterpart from Torulopsis utilis (87%).

摘要

测定了酿酒酵母（Saccharomyces cerevisiae）的异亮氨酸tRNA（tRNAIle）的核苷酸序列。其一级结构为：pG-G-U-C-U-C-U-U-m1G-m2G-C-C-C-A-G-D-D-G-G-D-D-A-A-G-G-C-A-C-C-G-U-G-C-U-I-A-U-t6A-A-C-G-C-G-G-G-GA-D-m5C-A-G-C-G-G-T-ψ-C-G-m1A-U-C-C-C-G-C-U-A-G-A-G-A-C-C-A-C-C-A。其反密码子为I-A-U。因此它应该能识别三种异亮氨酸密码子，也正因为如此，它可能是酿酒酵母中异亮氨酸唯一的同功受体tRNA。它与其来自产朊假丝酵母（Torulopsis utilis）的对应物具有高度同源性（87%）。