Sternby B, Borgström B
Biochim Biophys Acta. 1984 Apr 27;786(1-2):109-12. doi: 10.1016/0167-4838(84)90160-2.
Purified antibodies to human colipase86 were coupled to CNBr-activated Sepharose 4B. The immunoadsorption column thus obtained was used to purify procolipase from human pancreatic juice in one step by immunoaffinity chromatography. A single form of procolipase was obtained, having similar biological properties as previously characterized procolipases from horse and pig. The sequence of the N-terminal propeptide was determined to be Ala-Pro-Gly-Pro-Arg. In bovine, equine and porcine procolipases the corresponding N-terminal sequence is Val-Pro-Asp-Pro-Arg.
将人辅脂酶86的纯化抗体偶联到溴化氰活化的琼脂糖4B上。由此获得的免疫吸附柱用于通过免疫亲和色谱一步从人胰液中纯化前辅脂酶。获得了一种单一形式的前辅脂酶，其生物学特性与先前鉴定的来自马和猪的前辅脂酶相似。确定N端前肽的序列为丙氨酸-脯氨酸-甘氨酸-脯氨酸-精氨酸。在牛、马和猪的前辅脂酶中，相应的N端序列是缬氨酸-脯氨酸-天冬氨酸-脯氨酸-精氨酸。
