Bosc-Bierne I, Rathelot J, Bechis G, Delori P, Sarda L
Biochimie. 1984 May;66(5):413-6. doi: 10.1016/0300-9084(84)90025-7.
Purified antibodies raised against chicken colipase were coupled to Sepharose 4B and colipase was isolated in a single step by immunoaffinity chromatography from an extract of chicken pancreas prepared under conditions where trypsin activation is avoided. The purified protein has a single amino terminal residue of alanine and its biochemical properties are similar to those of the precursor form of colipase (procolipase) previously isolated from porcine and equine pancreas or pancreatic juice. Further evidence for the existence of procolipase was obtained from kinetic studies of the hydrolysis of the Intralipid emulsion by untreated and trypsin-treated chicken pancreatic juice.
针对鸡辅脂酶产生的纯化抗体与琼脂糖4B偶联，通过免疫亲和色谱从避免胰蛋白酶激活的条件下制备的鸡胰腺提取物中一步分离出辅脂酶。纯化的蛋白质具有单个丙氨酸氨基末端残基，其生化特性与先前从猪和马的胰腺或胰液中分离出的辅脂酶前体（前辅脂酶）相似。通过未处理和经胰蛋白酶处理的鸡胰液对英脱利匹特乳剂水解的动力学研究，获得了前辅脂酶存在的进一步证据。
