Lautenschlager I, Taskinen E, Inkinen K, Lehto V P, Virtanen I, Häyry P
Cell Immunol. 1984 Apr 15;85(1):191-200. doi: 10.1016/0008-8749(84)90289-2.
Monoclonal mouse antibodies to the "framework" determinants of the class I and II molecules of the major histocompatibility complex (MHC) were used to demonstrate the presence of the MHC antigens in human liver. First, the localization of these antigens was demonstrated from frozen section histology with indirect FITC immunofluorescence and the cell component(s) binding the mouse antibody were identified by rabbit marker antisera and indirect TRITC immunofluorescence. Second, the antigen expression on the cell surface was analyzed by the Staphylococcus aureus rosette method from cytological cell smears. All antibodies reacted with cells in the liver sinusoids, both with the Kupffer cells and at least partially with the sinusoidal endothelial cells. The same antisera reacted also with the bile duct cells, though weaker, and with some stromal cells in close proximity of the blood vessels. The vascular endothelial cells of hepatic artery, hepatic vein, and portal vein displayed no reaction. Thus human liver differs strikingly from, e.g., human kidney, where the vascular endothelial cells contain large amounts of MHC antigens on the cell surface. This difference may be one explanation to why liver allografts are less promptly rejected than renal allografts in man.
利用针对主要组织相容性复合体（MHC）I类和II类分子“构架”决定簇的单克隆小鼠抗体，来证明人类肝脏中MHC抗原的存在。首先，通过间接FITC免疫荧光从冰冻切片组织学中证明这些抗原的定位，并通过兔标记抗血清和间接TRITC免疫荧光鉴定与小鼠抗体结合的细胞成分。其次，通过细胞学涂片的金黄色葡萄球菌花环法分析细胞表面的抗原表达。所有抗体均与肝血窦中的细胞发生反应，包括库普弗细胞以及至少部分与窦状内皮细胞发生反应。相同的抗血清也与胆管细胞发生反应，不过反应较弱，并且与血管附近的一些基质细胞发生反应。肝动脉、肝静脉和门静脉的血管内皮细胞未显示反应。因此，人类肝脏与例如人类肾脏显著不同，在人类肾脏中，血管内皮细胞在细胞表面含有大量MHC抗原。这种差异可能是人类肝脏同种异体移植比肾脏同种异体移植排斥反应更慢的一种解释。
