乳腺肿瘤细胞膜上HLA-D/DR抗原的存在。
Presence of HLA-D/DR antigens on the membrane of breast tumour cells.
作者信息
Bernard D, Maurizis J C, Rusé F, Chassagne J, Chollet P, Sauvezie B, de Latour M, Plagne R
出版信息
Clin Exp Immunol. 1984 Apr;56(1):215-21.
HLA-D/DR (Ia) glycoproteins were identified in human breast carcinoma and normal mammary gland cells by means of an anti-Ia monoclonal antibody. Two techniques were used: (1) immunoperoxidase staining performed on histological sections and (2) Ia glycoproteins were isolated as follows: firstly by radioactive labelling of isolated cells, then by filtration on Sephadex G25, followed by Lens culinaris chromatography, and immune complex formation and then elution on protein A-Sepharose. Lastly, the immune complex was studied by chromatofocusing. Both techniques revealed that Ia expression was found in carcinoma cells, but not in normal cells.
通过抗Ia单克隆抗体在人乳腺癌细胞和正常乳腺细胞中鉴定出HLA - D/DR（Ia）糖蛋白。采用了两种技术：（1）对组织切片进行免疫过氧化物酶染色；（2）Ia糖蛋白的分离如下：首先对分离的细胞进行放射性标记，然后通过Sephadex G25过滤，接着进行扁豆凝集素层析、免疫复合物形成，随后在蛋白A - 琼脂糖上洗脱。最后，通过色谱聚焦研究免疫复合物。两种技术均显示Ia表达见于癌细胞，而不见于正常细胞。
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