Bernard D J, Maurizis J C, Chassagne J, Chollet P, Plagne R
Cancer Res. 1985 Mar;45(3):1152-8.
Class II HLA antigen expression in breast carcinoma and normal breast gland cells was compared using a method more accurate than immunofluorescence. This new method involves labeling membrane proteins with 131I and the anti-Class II HLA monoclonal antibody with 125I. The isolation and purification of the doubly labeled (125I-131I) immune complex was performed by affinity chromatography and chromatofocusing successively. When the specific activity of glycoproteins is known, the amount of glycoproteins which bind specifically to the anti-Class II HLA monoclonal antibody can be deduced. In breast carcinoma cells, 1.5 to 2% of the purified glycoproteins bind specifically to the monoclonal antibody, whereas less than 0.3% of normal breast gland cells binds. In contrast, leukemic cells, of which 80 to 90% possess Class II HLA antigens, 2 to 3% of Class II HLA glycoproteins bind specifically with the anti-Class II HLA monoclonal antibody.
使用一种比免疫荧光更精确的方法，比较了乳腺癌细胞和正常乳腺细胞中II类HLA抗原的表达。这种新方法包括用131I标记膜蛋白，用125I标记抗II类HLA单克隆抗体。通过亲和色谱和色谱聚焦依次进行双标记（125I-131I）免疫复合物的分离和纯化。当糖蛋白的比活性已知时，可以推断出与抗II类HLA单克隆抗体特异性结合的糖蛋白量。在乳腺癌细胞中，1.5%至2%的纯化糖蛋白与单克隆抗体特异性结合，而正常乳腺细胞的这一比例不到0.3%。相比之下，80%至90%的白血病细胞具有II类HLA抗原，2%至3%的II类HLA糖蛋白与抗II类HLA单克隆抗体特异性结合。
