Kamarck M E, Barker P E, Miller R L, Ruddle F H
Exp Cell Res. 1984 May;152(1):1-14. doi: 10.1016/0014-4827(84)90224-6.
The recent advances in human gene mapping have been largely due to the development of interspecies cell hybrids containing human chromosomes and their fragments. The importance of characterized panels of these hybrid lines has grown exponentially with the application of recombinant DNA technologies to human genetics. In this article, we discuss current strategies employed in the construction of somatic cell hybrid mapping panels.
人类基因图谱绘制的近期进展在很大程度上归功于含有人类染色体及其片段的种间细胞杂种的发展。随着重组DNA技术在人类遗传学中的应用，这些杂种细胞系特征明确的细胞株的重要性呈指数级增长。在本文中，我们讨论了构建体细胞杂种定位细胞株所采用的当前策略。
