Brook J D, Shaw D J, Thomas N S, Meredith A L, Cowell J, Harper P S
Cytogenet Cell Genet. 1986;41(1):30-7. doi: 10.1159/000132192.
A series of mouse-human somatic cell hybrid lines (WILF) were derived from a hybrid that was originally thought to have chromosome 19 as its only human chromosome. In situ hybridization has been used to assess the amount of human material present in the different lines. All appear to contain different numbers of human chromosome fragments. A series of X-chromosome-specific DNA sequences hybridized against DNA from the lines revealed that material from the X long arm is present in several cases. Chromosome 19-specific DNA sequences used in a similar way show that fragmentation of this this chromosome has occurred with subsequent segregation of the fragments in different lines. The localization of these markers to various regions of chromosome 19, and their relation to the fragments observed in the WILF lines, is discussed.
一系列小鼠 - 人类体细胞杂交系（WILF）源自一个最初被认为仅含人类19号染色体的杂交细胞。原位杂交已用于评估不同细胞系中人类物质的含量。所有细胞系似乎都含有不同数量的人类染色体片段。一系列X染色体特异性DNA序列与这些细胞系的DNA杂交显示，在一些情况下存在来自X染色体长臂的物质。以类似方式使用的19号染色体特异性DNA序列表明，这条染色体发生了片段化，随后片段在不同细胞系中分离。本文讨论了这些标记物在19号染色体各个区域的定位，以及它们与在WILF细胞系中观察到的片段的关系。
