癌症放射免疫检测中放射性标记抗体的第二抗体清除
Second antibody clearance of radiolabeled antibody in cancer radioimmunodetection.
作者信息
Sharkey R M, Primus F J, Goldenberg D M
出版信息
Proc Natl Acad Sci U S A. 1984 May;81(9):2843-6. doi: 10.1073/pnas.81.9.2843.
Abstract
The imaging of tumors using radiolabeled antibodies previously has required the implementation of computer-assisted subtraction techniques to reduce background radioactivity. A decrease in radioactivity in the blood of hamsters bearing human colonic tumor xenografts has been achieved by administering a second antibody directed against a radiolabeled primary antibody to carcinoembryonic antigen (CEA). This method was found to reduce the level of blood radioactivity by a factor of 4 within 2 hr after injection of the second antibody and to enhance tumor/nontumor ratios within 24 hr. Unlike liposomally entrapped second antibody, the primary anti-CEA antibody did not show increased accretion of radioactivity in the liver, spleen, or other major organs. These results suggest that administration of a second antibody alone may improve tumor imaging with a radiolabeled antitumor antibody.
摘要
此前，使用放射性标记抗体对肿瘤进行成像需要采用计算机辅助减法技术来降低背景放射性。通过给携带人结肠肿瘤异种移植瘤的仓鼠注射第二种针对放射性标记的癌胚抗原（CEA）一抗的抗体，已实现了仓鼠血液中放射性的降低。发现该方法在注射第二种抗体后2小时内可将血液放射性水平降低4倍，并在24小时内提高肿瘤/非肿瘤比值。与脂质体包裹的第二种抗体不同，一抗CEA抗体在肝脏、脾脏或其他主要器官中未显示放射性积聚增加。这些结果表明，单独给予第二种抗体可能会改善放射性标记抗肿瘤抗体的肿瘤成像效果。
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