Krensky A M, Sanchez-Madrid F, Springer T A, Burakoff S J
Surv Immunol Res. 1984;3(1):39-44. doi: 10.1007/BF02918596.
Three cell surface molecules, designated LFA-1, LFA-2, and LFA-3 were identified by mAbs selected for their ability to block cytolysis by an OKT4+, HLA-DR-specific CTL line. The LFA mAbs block all CTL and proliferative functions studied. In addition, anti-LFA-1 mAbs inhibit NK-mediated cytolysis. By analogy with murine LFA-1, human LFA-1 may be involved in the adhesion stage of cellular interactions. LFA-2, the SRBC receptor molecule, appears to be a T cell function-specific molecule. We have not yet established whether LFA-2 participates in antigen recognition or whether it is involved in antigen-non-specific interactions. The anti-LFA-3 mAb specifically blocks function by binding to the target cells, implying that LFA-3 may be a target ligand for an effector-specific receptor. The CTL-target interaction involves a number of steps, including antigen recognition, cell adhesion, and delivery of the lethal hit [22]. The LFA antigens show the complexity of this process at the molecular level. The anti-LFA monoclonal antibodies will be useful probes into the T cell immune response and may prove clinically relevant, both diagnostically and therapeutically.
通过选择具有阻断OKT4 +、HLA - DR特异性CTL系细胞溶解能力的单克隆抗体，鉴定出三种细胞表面分子，分别命名为淋巴细胞功能相关抗原-1（LFA - 1）、淋巴细胞功能相关抗原-2（LFA - 2）和淋巴细胞功能相关抗原-3（LFA - 3）。LFA单克隆抗体可阻断所研究的所有CTL和增殖功能。此外，抗LFA - 1单克隆抗体可抑制自然杀伤细胞（NK）介导的细胞溶解。与小鼠LFA - 1类似，人LFA - 1可能参与细胞相互作用的黏附阶段。LFA - 2即绵羊红细胞（SRBC）受体分子，似乎是一种T细胞功能特异性分子。我们尚未确定LFA - 2是否参与抗原识别，或者它是否参与抗原非特异性相互作用。抗LFA - 3单克隆抗体通过与靶细胞结合特异性阻断功能，这意味着LFA - 3可能是效应细胞特异性受体的靶配体。CTL与靶细胞的相互作用涉及多个步骤, 包括抗原识别、细胞黏附以及致命一击的传递[22]。LFA抗原在分子水平上显示了这一过程的复杂性。抗LFA单克隆抗体将成为研究T细胞免疫反应的有用探针，并且可能在诊断和治疗方面都具有临床相关性。
