Kürzinger K, Reynolds T, Germain R N, Davignon D, Martz E, Springer T A
J Immunol. 1981 Aug;127(2):596-602.
We have previously described a monoclonal antibody (MAb), M7/14, which blocks a variety of T cell functions, including CTL-mediated killing, the mixed lymphocyte response, and antigen-specific proliferation. The antigen defined by M7/14 has been designated lymphocyte function-associated antigen one (LFA-1). In this report, LFA-1 has been studied as to cell distribution, surface abundance, structure, and in comparison to other CTL surface antigens, LFA-1 is expressed on lymphoid cells of both the T and the B lineages and on a large fraction of bone marrow cells, but not on exudate macrophages or nonlymphoid tissues. T cells express more LFA-1 than B cells, both in the unstimulated and stimulated states. Compared with unstimulated spleen cells, cytolytic T lymphocyte cell preparations (CTLP) and Con A blasts, but not LPS blasts, show increased LFA-1 expression relative to H-2, and for T cell-containing populations, Lyt-2. M7/14 MAb binds to about 1.5 X 10(4) and 7 X 10(4) LFA-1 sites per average spleen cell or CTLP cell, respectively. M7/14 Mab binds to cTLP in quantitites of 2.5-fold and ies 10.4-fold less than H-2 and Thy-1 Mab, respectively; since the latter have little or no effect on CTL function, inhibition of killing by M7/14 MAb is specific for the LFA-1 surface site. M7/14 MAb and a blocking Lyt-2 MAb are bound in similar quantities of CTLP. LFA-1 is a glycoprotein and consists of 2 noncovalently linked polypeptide chains of 180,000 and 95,000 Mr. The same molecular species as on CTL is present on other T cells and on B cells. The molecular structure and cell distribution of LFA-1 clearly distinguishes it from Lyt-2,3, Ly-5, T145, and T11, which were previously suggested to be either associated with the function of and/or present on the surface of CTL.
我们之前描述过一种单克隆抗体（MAb），即M7/14，它能阻断多种T细胞功能，包括细胞毒性T淋巴细胞（CTL）介导的杀伤作用、混合淋巴细胞反应以及抗原特异性增殖。由M7/14所界定的抗原已被命名为淋巴细胞功能相关抗原-1（LFA-1）。在本报告中，对LFA-1的细胞分布、表面丰度、结构进行了研究，并且与其他CTL表面抗原相比较，LFA-1在T和B淋巴细胞系的淋巴样细胞以及大部分骨髓细胞上表达，但在渗出液巨噬细胞或非淋巴组织上不表达。在未受刺激和受刺激状态下，T细胞表达的LFA-1均多于B细胞。与未受刺激的脾细胞相比，细胞毒性T淋巴细胞制剂（CTLP）和刀豆蛋白A刺激的母细胞，但不是脂多糖刺激的母细胞，相对于H-2显示出LFA-1表达增加，对于含T细胞群体，相对于Lyt-2也是如此。M7/14单克隆抗体分别与每个平均脾细胞或CTLP细胞上约1.5×10⁴和7×10⁴个LFA-1位点结合。M7/14单克隆抗体与cTLP的结合量分别比H-2和Thy-1单克隆抗体少2.5倍和10.4倍；由于后者对CTL功能几乎没有影响，所以M7/14单克隆抗体对杀伤作用的抑制作用对LFA-1表面位点具有特异性。M7/14单克隆抗体和一种阻断Lyt-2的单克隆抗体与CTLP的结合量相似。LFA-1是一种糖蛋白，由两条非共价连接的分子量分别为180,000和95,000的多肽链组成。与CTL上相同的分子种类也存在于其他T细胞和B细胞上。LFA-1的分子结构和细胞分布使其明显区别于Lyt-2、3、Ly-5、T145和T11，这些分子之前被认为要么与CTL的功能相关，要么存在于CTL表面。
