Nederman T, Norling B, Glimelius B, Carlsson J, Brunk U
Cancer Res. 1984 Jul;44(7):3090-7.
Multicellular spheroids of a human glioma cell line (U-118 MG) and a human thyroid cancer cell line ( HTh -7) were analyzed for the presence of extracellular matrix (ECM) using light microscopy, transmission electron microscopy, and indirect immunofluorescence staining for fibronectin, laminin, and collagen. These studies were supplemented by analyses of glycosaminoglycans using autoradiography or chemical methods after metabolic labeling with [35S]sulfate or [3H]glucosamine in conjunction with various extraction procedures. The results showed that both types of spheroids contained an ECM composed of fibronectin, laminin, collagen, and glycosaminoglycans. The organization of the ECM in the spheroids seemed to be similar to that of tumors in vivo. These findings help justify the use of the spheroid system as an in vitro model for the study of biological phenomena of human tumors in vivo. Furthermore, it is concluded that the formation of an ECM in vitro is not confined to normal cells but can be promoted in transformed cells using appropriate culture conditions.
利用光学显微镜、透射电子显微镜以及针对纤连蛋白、层粘连蛋白和胶原蛋白的间接免疫荧光染色，分析了人胶质瘤细胞系（U - 118 MG）和人甲状腺癌细胞系（HTh - 7）的多细胞球体中细胞外基质（ECM）的存在情况。在用[35S]硫酸盐或[3H]葡糖胺进行代谢标记并结合各种提取程序后，通过放射自显影或化学方法对糖胺聚糖进行分析，对这些研究进行了补充。结果表明，两种类型的球体均含有由纤连蛋白、层粘连蛋白、胶原蛋白和糖胺聚糖组成的细胞外基质。球体中细胞外基质的组织方式似乎与体内肿瘤的相似。这些发现有助于证明将球体系统用作研究体内人类肿瘤生物学现象的体外模型的合理性。此外，得出的结论是，体外细胞外基质的形成并不局限于正常细胞，而是可以通过适当的培养条件在转化细胞中促进其形成。
