使用维持最佳培养条件的MRI装置对细胞聚集体进行定量表征的方案。

Wißmann Rebecca, Martirosian Petros, Danalache Marina, Elser Stefanie, Schick Fritz

Department of Diagnostic and Interventional Radiology, University Hospital of Tübingen, 72076 Tübingen, Germany.

Department of Diagnostic and Interventional Radiology, University Hospital of Tübingen, 72076 Tübingen, Germany; Section on Experimental Radiology, Department of Diagnostic and Interventional Radiology, Tübingen University Hospital, Tübingen, Germany.

STAR Protoc. 2025 Jul 30;6(3):104002. doi: 10.1016/j.xpro.2025.104002.

The use of destructive biochemical assays and the preparation of histologic samples are routinely employed to monitor development and viability of 3D cell aggregates. Magnetic resonance imaging (MRI) offers a non-destructive, high-resolution alternative to histological analysis, enabling longitudinal assessment of cellular dynamics while preserving sample integrity. Here, we present a protocol for non-invasive MR imaging of cell spheroid clusters by creating an adequate imaging environment. We describe steps for spheroid formation, casting of the imaging tube, cell cultivation, and data evaluation. For complete details on the use and execution of this protocol, please refer to Wißmann et al..

使用破坏性生化分析和制备组织学样本通常用于监测3D细胞聚集体的发育和活力。磁共振成像（MRI）为组织学分析提供了一种非破坏性、高分辨率的替代方法，能够在保持样本完整性的同时对细胞动态进行纵向评估。在这里，我们通过创建一个合适的成像环境，提出了一种用于细胞球体簇无创磁共振成像的方案。我们描述了球体形成、成像管铸造、细胞培养和数据评估的步骤。有关此方案的使用和执行的完整详细信息，请参考维茨曼等人的研究。