Huth A, Rapoport T A, Kääriäinen L
EMBO J. 1984 Apr;3(4):767-71. doi: 10.1002/j.1460-2075.1984.tb01882.x.
The mRNA coding for the structural proteins of Semliki Forest virus, the 26S RNA, was injected into Xenopus oocytes. Synthesis of the capsid protein and the three envelope glycoproteins E1, E2 and E2 was observed. The proteins, which are normally incorporated into the plasma membrane of infected cells, are transported to the surface of the oocytes. The transport of the membrane proteins takes place in the presence of tunicamycin. The results show that the proteins foreign to the oocyte reach their destination in the plasma membrane. Consequently, the mRNA contains the information for the transport and proteolytic cleavage of the polypeptides.
编码塞姆利基森林病毒结构蛋白的信使核糖核酸（mRNA），即26S RNA，被注入非洲爪蟾卵母细胞中。观察到衣壳蛋白以及三种包膜糖蛋白E1、E2和E2的合成。这些通常会整合到被感染细胞质膜中的蛋白质被转运到卵母细胞表面。膜蛋白的转运在衣霉素存在的情况下发生。结果表明，卵母细胞外来的蛋白质到达了质膜中的目的地。因此，mRNA包含了多肽转运和蛋白水解切割的信息。
