Snyder A K, Singh S P
Horm Metab Res. 1984 Apr;16(4):175-8. doi: 10.1055/s-2007-1014736.
Insulin binding to liver membranes has been studied in term fetuses of rats fed ethanol-containing liquid diet during pregnancy . Pair-fed and ad libitum-fed controls received liquid diet in which maltose-dextrins were substituted isocalorically for ethanol. Food consumption and body weigh gain of ethanol- imbibing dams were 35% and 70% less than their ad libitum counterparts respectively. Ethanol-fed rats also exhibited less gain in body weight than pair-fed controls despite isocalorically equivalent food intake. The number of live pups was not different among the various groups; however, liver weight of fetuses exposed to ethanol in utero was 47% less than those of the pups of ad libitum control dams and 28% less than those of the offspring of pair-fed control rats. Insulin binding to liver membranes of fetuses exposed to ethanol in utero was lower than that of ad libitum controls but was not significantly different from that of the pair-fed control animals. Average affinity profiles showed a reduction in K at all levels of receptor occupancy in the fetuses of ethanol-fed rats. For fetuses of the pair-fed group, K was reduced only at fractional occupancy below 20% but not at higher fractional occupancy. Because of the similarity of insulin binding in the fetuses of the ethanol-fed rats and their pair-fed counterparts, effects of ethanol on insulin binding cannot account for the reduced hepatic glycogen stores previously reported in term fetuses.
对孕期摄入含乙醇液体饲料的大鼠足月胎儿的肝细胞膜胰岛素结合情况进行了研究。配对喂养和自由进食对照组接受的液体饲料中，麦芽糖糊精等量替代了乙醇。摄入乙醇的母鼠的食物消耗量和体重增加量分别比自由进食的母鼠少35%和70%。尽管摄入的热量相当，但摄入乙醇的大鼠的体重增加也比配对喂养的对照组少。各组的活仔数没有差异；然而，子宫内接触乙醇的胎儿的肝脏重量比自由进食对照组母鼠的幼崽少47%，比配对喂养对照组大鼠的后代少28%。子宫内接触乙醇的胎儿的肝细胞膜胰岛素结合低于自由进食对照组，但与配对喂养对照动物无显著差异。平均亲和力曲线显示，摄入乙醇大鼠的胎儿在所有受体占有率水平下K值均降低。对于配对喂养组的胎儿，仅在占有率低于20%时K值降低，而在较高占有率时则没有降低。由于摄入乙醇大鼠的胎儿与其配对喂养对应物的胰岛素结合情况相似，乙醇对胰岛素结合的影响不能解释先前报道的足月胎儿肝糖原储备减少的情况。
