Tsokos M, Linnoila R I, Chandra R S, Triche T J
Hum Pathol. 1984 Jun;15(6):575-84. doi: 10.1016/s0046-8177(84)80012-x.
Immunocytochemical staining for neuron-specific enolase (NSE) was performed in 44 round-cell tumors from children by the improved immunoglobulin-enzyme bridge method with antiserum against NSE. The tumors studied consisted of 15 neuroblastomas showing various degrees of histologic differentiation, 13 Ewing's sarcomas, ten soft tissue sarcomas of diverse origin, and six lymphomas of bone and soft tissues. Neuron-specific enolase was detected in all neuroblastomas, irrespective of the degree of histologic differentiation. None of the other round-cell tumors was positive for NSE, except one embryonal rhabdomyosarcoma that contained differentiated myoblasts. The primitive cells of this tumor were negative as well. It is concluded that immunocytochemical staining with antibodies to NSE is a practical and reliable method for distinguishing neuroblastomas from other nonneural round-cell tumors in children. This is true even for the most primitive forms of neuroblastomas, in which morphologic techniques are less reliable. Neuron-specific enolase may also be useful in delineating the neural histogenesis of other ill-defined tumors.
采用抗神经元特异性烯醇化酶（NSE）抗血清，通过改良的免疫球蛋白 - 酶桥法，对44例儿童圆形细胞瘤进行了免疫细胞化学染色。所研究的肿瘤包括15例显示不同程度组织学分化的神经母细胞瘤、13例尤因肉瘤、10例不同起源的软组织肉瘤以及6例骨和软组织淋巴瘤。在所有神经母细胞瘤中均检测到神经元特异性烯醇化酶，无论其组织学分化程度如何。除了1例含有分化成肌细胞的胚胎性横纹肌肉瘤外，其他圆形细胞瘤均未检测到NSE阳性。该肿瘤的原始细胞也呈阴性。结论是，用抗NSE抗体进行免疫细胞化学染色是区分儿童神经母细胞瘤与其他非神经圆形细胞瘤的实用且可靠的方法。即使对于形态学技术不太可靠的最原始形式的神经母细胞瘤，情况也是如此。神经元特异性烯醇化酶在界定其他不明肿瘤的神经组织发生方面可能也有用。
