Booth R J, Pang G T, Watson J D
J Biol Response Mod. 1984;3(2):206-18.
The involvement of interleukin-2 (IL-2) in the generation of human antibody responses was studied using the pokeweed mitogen (PWM)-induced sheep erythrocyte-specific plaque-forming cell (SE-PFC) response of cultured tonsilar lymphocytes. IL-2-containing supernatants from phytohemagglutinin-stimulated human spleen or tonsil cells or the cloned human T cell line JURKAT were capable of replacing the requirement for PWM in the generation of SE-PFC and resulted in a more prolonged response than that observed with PWM. This effect of IL-2-containing medium was dependent on the presence of SE-derived antigens and was not due to the induction of polyclonal antibody synthesis. When purified by gel filtration the SE-PFC-inducing activity co-eluted with IL-2 with an apparent molecular weight of approximately 15,000 (range, 8,000-20,000). Selective removal of IL-2 from crude or partially purified IL-2 preparations by extensive adsorption with activated murine thymocytes or an IL-2-dependent T cell line completely eliminated SE-PFC-inducing activity, indicating that IL-2 itself was necessary for the effect. The question of whether or not this effect was mediated through T cells is discussed.
利用培养的扁桃体淋巴细胞对商陆有丝分裂原（PWM）诱导的绵羊红细胞特异性空斑形成细胞（SE-PFC）反应，研究了白细胞介素-2（IL-2）在人类抗体反应产生中的作用。来自植物血凝素刺激的人脾或扁桃体细胞或克隆的人T细胞系JURKAT的含IL-2的上清液能够替代PWM在SE-PFC产生中的需求，并导致比PWM观察到的更持久的反应。含IL-2培养基的这种作用取决于SE衍生抗原的存在，而不是由于多克隆抗体合成的诱导。通过凝胶过滤纯化时，SE-PFC诱导活性与IL-2共洗脱，表观分子量约为15,000（范围为8,000-20,000）。用活化的小鼠胸腺细胞或IL-2依赖性T细胞系广泛吸附从粗制或部分纯化的IL-2制剂中选择性去除IL-2，完全消除了SE-PFC诱导活性，表明IL-2本身对该作用是必需的。讨论了这种作用是否通过T细胞介导的问题。
