Fauci A S, Pratt K R, Whalen G
J Immunol. 1976 Dec;117(6):2100-4.
A recently described system for the induction and assay of plaque-forming cells (PFC) following polyclonal stimulation of human B lymphocytes was employed to delineate the cellular requirements for pokeweed mitogen (PWM)-induced activation of human tonsillar and peripheral blood B lymphocytes. It was found that the process was not dependent on the presence of monocytes, but was dependent on the presence of T cells. T cell depleted suspensions of tonsillar as well as peripheral blood lymphocytes had markedly diminished if not absent PFC responses following PWM stimulation. The PFC responses of T cell depleted cultures of tonsil and blood could be reconstituted by adding back T cells. T cell supernatants could also reconstitute the PFC response of T cell depleted tonsillar lymphocytes but not of T cell depleted blood lymphocytes, thus suggesting a more stringent T cell requirement of the PWM-induced PFC response of blood lymphocytes. Enrichment for T cells on the other hand enhanced PFC responses in both organs, particularly in those cultures which had low responses in unfractionated suspensions demonstrating the requirement of optimal T cell help for maximal PFC responses. This study demonstrates the feasibility and effectiveness of this PFC system in elucidating some of the complex regulatory processes associated with the activation of human B lymphocytes.
一种最近描述的用于在人B淋巴细胞多克隆刺激后诱导和检测形成空斑细胞（PFC）的系统，被用于确定美洲商陆丝裂原（PWM）诱导人扁桃体和外周血B淋巴细胞活化所需的细胞条件。结果发现，该过程不依赖于单核细胞的存在，但依赖于T细胞的存在。扁桃体以及外周血淋巴细胞的T细胞耗竭悬液在PWM刺激后，即使没有完全缺失，其PFC反应也明显减弱。通过回加T细胞，可以重建扁桃体和血液中T细胞耗竭培养物的PFC反应。T细胞上清液也可以重建扁桃体T细胞耗竭淋巴细胞的PFC反应，但不能重建外周血T细胞耗竭淋巴细胞的PFC反应，这表明外周血淋巴细胞的PWM诱导的PFC反应对T细胞的要求更为严格。另一方面，T细胞富集增强了两个器官中的PFC反应，特别是在那些未分级悬液中反应较低的培养物中，这表明最大的PFC反应需要最佳的T细胞辅助。这项研究证明了该PFC系统在阐明一些与人类B淋巴细胞活化相关的复杂调节过程中的可行性和有效性。
