Kurl D N, Christensen K K, Christensen P
J Med Microbiol. 1984 Jun;17(3):347-51. doi: 10.1099/00222615-17-3-347.
Nine strains of group B streptococci type III, five with R-protein (R+) and four without (R-) were tested for capacity to colonise the upper respiratory tract in mice and to adhere to human buccal cells. In the mouse model, 80-microliter inocula of dilutions of overnight cultures of the strains in Todd Hewitt broth were placed in the external nares under light ether anaesthesia. A pilot experiment demonstrated that it was reasonable to study the throat colonisation 2 and 4 days after inoculation. Groups of 18-20 mice were then given inocula containing 8 X 10(6) cfu/ml of five R+ and four R- strains. At day 4, significantly more mice were colonised with type III, R+ strains (73% of the animals) than with type III, R- strains (44%) (p less than 0.01). In adherence experiments with human buccal cells, no difference was found between the R+ and R- strains. The results indicated that the higher colonisation rate among R+ strains was mediated by mechanisms other than adherence.
对9株III型B族链球菌进行了测试，其中5株带有R蛋白（R+），4株没有（R-），检测它们在小鼠上呼吸道定殖的能力以及对人颊细胞的黏附能力。在小鼠模型中，在轻度乙醚麻醉下，将菌株在托德-休伊特肉汤中过夜培养物的稀释液80微升接种到外鼻孔。一项预实验表明，在接种后2天和4天研究咽喉定殖情况是合理的。然后给18 - 20只小鼠组成的几组接种含有5株R+菌株和4株R-菌株、浓度为8×10⁶ cfu/ml的菌液。在第4天，III型R+菌株定殖的小鼠（占动物的73%）显著多于III型R-菌株定殖的小鼠（44%）（p<0.01）。在与人颊细胞的黏附实验中，R+菌株和R-菌株之间未发现差异。结果表明，R+菌株中较高的定殖率是由黏附以外的机制介导的。
