从大肠杆菌DNA中去除紫外线诱导产生的嘧啶 - 嘧啶酮（6-4）产物需要uvrA、uvrB和uvrC基因产物。
Removal of UV light-induced pyrimidine-pyrimidone(6-4) products from Escherichia coli DNA requires the uvrA, uvrB, and urvC gene products.
作者信息
Franklin W A, Haseltine W A
出版信息
Proc Natl Acad Sci U S A. 1984 Jun;81(12):3821-4. doi: 10.1073/pnas.81.12.3821.
Abstract
Ultraviolet light induces the formation of cyclobutane pyrimidine dimers and pyrimidine- pyrimidone (6-4) photoproducts in cellular DNA. In Escherichia coli, the uvrA, uvrB, and uvrC genes are necessary for excision of cyclobutane dimers. To determine whether the uvrABC gene products are required for (6-4) product removal from DNA, a sensitive HPLC assay was developed that allows the separation and quantitation of both types of photoproducts. Both the T T cyclobutane dimer and the T-C(6-4) product were completely removed from the DNA after 2 hr of repair in a wild-type strain. Both products were also removed in the wild-type strain in the presence of chloramphenicol, an inhibitor of protein synthesis. No decrease in the amount of either T T cyclobutane dimer or of T-C(6-4) products was observed in strains that were deficient in any one of the three uvr gene products under similar conditions. We conclude the uvrABC enzyme complex is required for excision of (6-4) photoproducts from E. coli DNA.
摘要
紫外线可诱导细胞DNA中形成环丁烷嘧啶二聚体和嘧啶 - 嘧啶酮（6 - 4）光产物。在大肠杆菌中，uvrA、uvrB和uvrC基因对于切除环丁烷二聚体是必需的。为了确定uvrABC基因产物是否是从DNA中去除（6 - 4）产物所必需的，开发了一种灵敏的高效液相色谱分析法，该方法能够分离并定量这两种类型的光产物。在野生型菌株中修复2小时后，T - T环丁烷二聚体和T - C（6 - 4）产物都从DNA中被完全去除。在存在蛋白质合成抑制剂氯霉素的情况下，野生型菌株中的这两种产物也被去除。在类似条件下，在三种uvr基因产物中任何一种有缺陷的菌株中，未观察到T - T环丁烷二聚体或T - C（6 - 4）产物的量减少。我们得出结论，uvrABC酶复合物是从大肠杆菌DNA中切除（6 - 4）光产物所必需的。
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