LeBouton A V
Anat Rec. 1984 May;209(1):67-75. doi: 10.1002/ar.1092090109.
The indirect immunoperoxidase method was used to identify albumin in hepatocytes of young rats before and after periods of starvation and during a normal diurnal cycle. All liver cells in fed rats contained an abundance of albumin, whereas hepatocytes from overnight fasted animals showed minimal amounts of the protein. Hepatocytes in rats on the diurnal cycle generally contained more albumin during the light phase than in darkness. At the beginning of the dark phase, certain hepatocytes were low in albumin and they were located primarily around portal canals. Halfway through the dark period, these cells had increased in number and were located closer to terminal hepatic venules. Overnight starvation of young rats profoundly lowers hepatocyte albumin and the time of highest liver cell albumin content in the diurnal cycle of fed, young rats is during the first half of the light period.
采用间接免疫过氧化物酶法，对幼鼠在饥饿前后以及正常昼夜周期中肝细胞内的白蛋白进行鉴定。喂食大鼠的所有肝细胞都含有大量白蛋白，而禁食过夜动物的肝细胞中该蛋白含量极少。处于昼夜周期中的大鼠，其肝细胞通常在光照期比黑暗期含有更多白蛋白。在黑暗期开始时，某些肝细胞的白蛋白含量较低，且主要位于门静脉周围。在黑暗期过半时，这些细胞数量增加，且位置更靠近肝终末小静脉。幼鼠过夜禁食会显著降低肝细胞白蛋白含量，且在喂食的幼鼠昼夜周期中，肝细胞白蛋白含量最高的时间是在光照期的前半段。
