Frederiks W M, Marx F, Bosch K S, Van Noorden C J
University of Amsterdam, Academic Medical Centre, The Netherlands.
Histochemistry. 1987;87(5):439-43. doi: 10.1007/BF00496815.
The diurnal variation of 5'-nucleotidase activity in periportal and pericentral areas of rat liver parenchyma has been determined with quantitative histochemical means. 5'-Nucleotidase activity was estimated using microdensitometry in cryostat sections after being incubated with a medium according to Wachstein and Meisel (1957). It appeared that 5'-nucleotidase activity was significantly higher in pericentral areas than in periportal areas throughout the daily cycle and showed a maximum at the end of the light period. It was concluded that 5'-nucleotidase activity may be related with the capacity to diminish messenger RNA resulting in protein breakdown.
采用定量组织化学方法测定了大鼠肝实质门周区和中央周围区5'-核苷酸酶活性的昼夜变化。根据Wachstein和Meisel（1957年）的方法，在低温恒温器切片中与培养基孵育后，使用显微密度测定法估计5'-核苷酸酶活性。结果表明，在整个日常周期中，中央周围区的5'-核苷酸酶活性显著高于门周区，并在光照期结束时达到最大值。得出的结论是，5'-核苷酸酶活性可能与减少信使核糖核酸从而导致蛋白质分解的能力有关。
