采用酶免疫分析法测定大鼠肾组织中的氨基糖苷类药物。
Determination of aminoglycosides in rat renal tissue by enzyme immunoassay.
作者信息
Provoost A P, Van Schalkwijk W P, Adejuyigbe O, Van Leeuwen W B, Wagenvoort J H
出版信息
Antimicrob Agents Chemother. 1984 Apr;25(4):497-8. doi: 10.1128/AAC.25.4.497.
The enzyme-multiplied immunoassay technique for the determination of gentamicin or amikacin in serum was evaluated for use in rat renal tissue. After the addition of either gentamicin or amikacin to tissue homogenate, the assay of the supernatant showed a reduced recovery, depending on the amount of renal tissue present per milliliter of homogenate. The recovery was independent of the amount of aminoglycoside added. This reduction could be due to either protein binding or interference with the enzyme-multiplied immunoassay technique by substances released from the renal tissue. When the enzyme-multiplied immunoassay technique is used for aminoglycoside assay in renal tissue, care should be taken to minimize the amount of tissue present in the homogenate or to correct for the decreased recovery.
对用于测定血清中庆大霉素或阿米卡星的酶放大免疫测定技术在大鼠肾组织中的应用进行了评估。向组织匀浆中加入庆大霉素或阿米卡星后，对上清液的检测显示回收率降低，这取决于每毫升匀浆中肾组织的量。回收率与添加的氨基糖苷类药物的量无关。这种降低可能是由于蛋白质结合或肾组织释放的物质对酶放大免疫测定技术的干扰。当酶放大免疫测定技术用于肾组织中氨基糖苷类药物的检测时，应注意尽量减少匀浆中组织的量或对回收率降低进行校正。
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