Hsu C H, Kurtz T W, Weller J M
Antimicrob Agents Chemother. 1977 Aug;12(2):192-4. doi: 10.1128/AAC.12.2.192.
The mechanism of gentamicin uptake in vitro by renal cortical slices of rat kidney was investigated. The cortical-slice-uptake ratio of gentamicin concentration in 1.0 g of tissue water to that of 1.0 ml of incubation medium (SW/M) was 1.44 +/- 0.04. The uptake of gentamicin was inhibited by 2 x 10(-5) M dinitrophenol (SW/M = 1.03 +/- 0.04) and by anoxia (SW/M = 1.01 +/- 0.04). The results indicate that aerobic phosphorylation is required to transport gentamicin into the cells. The uptake of p-aminohippurate and tetraethylammonium chloride by renal cortical slices was not affected by gentamicin.
研究了庆大霉素在大鼠肾皮质切片中的体外摄取机制。庆大霉素在1.0 g组织水与1.0 ml孵育培养基中的皮质切片摄取浓度比（SW/M）为1.44±0.04。2×10⁻⁵ M二硝基苯酚（SW/M = 1.03±0.04）和缺氧（SW/M = 1.01±0.04）可抑制庆大霉素的摄取。结果表明，需有氧磷酸化才能将庆大霉素转运到细胞内。肾皮质切片对对氨基马尿酸和四乙氯化铵的摄取不受庆大霉素影响。
