在高温下于吖啶橙存在的情况下生长所诱导的肺炎克雷伯氏菌R质粒缺失
Deletions in Klebsiella pneumoniae R plasmids induced by growth in the presence of acridine orange at high temperature.
作者信息
Zorzópulos J, Trevisán A R, Denoya C D
出版信息
Antimicrob Agents Chemother. 1984 May;25(5):659-61. doi: 10.1128/AAC.25.5.659.
Abstract
The generation in vivo of plasmids deleted at specific sites in strains of Klebsiella pneumoniae containing R plasmids, by treatment with high concentrations of acridine orange (1.2 mg/ml) at 42 degrees C are reported. These deletions seem to be site specific because loss of specific restriction fragments after digestion with restriction enzymes was demonstrated.
摘要
据报道，在42℃下用高浓度吖啶橙（1.2mg/ml）处理含有R质粒的肺炎克雷伯菌菌株，可在体内产生特定位点缺失的质粒。这些缺失似乎具有位点特异性，因为用限制性内切酶消化后特定限制性片段的丢失得到了证实。
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