Richards K L, McCullough J
Immunol Commun. 1984;13(1):49-62. doi: 10.3109/08820138409025449.
A 48 well chemotaxis microchamber, originally designed for use with polycarbonate filters, was used with nitrocellulose filters to quantitate chemotaxis and chemokinesis of granulocytes. Various features of the microchamber were compared to Boyden chambers. The accuracy and reproducibility of the method were found to be comparable to Boyden chambers in the variability between individual readings and superior in the variability between replicate values. Concentrations of optimal doses of chemoattractant for chemotaxis and chemokinesis were similar using both types of chamber. The data indicates that this method may be useful in studying components of the chemotactic response which require the use of cellulose filters. Advantages of this method over standard Boyden chambers include the use of a single filter rather than individual, non-identical filters and a reduction in the number of cells required, particularly for pediatric testing or in neutropenic patients.
一种最初设计用于聚碳酸酯滤膜的48孔趋化性微室，被用于搭配硝酸纤维素滤膜来定量粒细胞的趋化性和趋化运动。该微室的各种特性与博伊登室进行了比较。结果发现，该方法在个体读数间的变异性方面与博伊登室相当，而在重复值间的变异性方面更优。使用这两种类型的室时，趋化性和趋化运动的趋化剂最佳剂量浓度相似。数据表明，该方法可能有助于研究需要使用纤维素滤膜的趋化反应成分。该方法相对于标准博伊登室的优势包括使用单个滤膜而非单独的、不相同的滤膜，以及所需细胞数量的减少，特别是在儿科检测或中性粒细胞减少患者中。
