Yayon A, Bauminger E R, Ofer S, Ginsburg H
J Biol Chem. 1984 Jul 10;259(13):8163-7.
Mössbauer studies of rat erythrocytes infected by Plasmodium berghei malaria parasites, using 57Fe-enriched rat red blood cells, were carried out in order to determine the physical parameters which characterize the malarial pigment iron and to test the effect of the widely used antimalaria drug, chloroquine, on these parameters. The iron in the malarial pigment which is derived from hemoglobin digestion by the intracellular parasite was found to be trivalent, high spin, with Mössbauer parameters which are significantly different from those of any known iron porphyrin containing compound. No difference was found between the parameters obtained in erythrocytes infected by drug-sensitive and drug-resistant strains of P. berghei, both before and after the treatment with chloroquine. The iron compound consists of microaggregates, about 30 A in diameter. These are somewhat larger in chloroquine-resistant strains and tend to increase in size in chloroquine-sensitive strains upon treatment with the drug. Mössbauer spectra of erythrocytes infected by a chloroquine-resistant strain revealed pigment iron in relative amounts invariable of those found in chloroquine-sensitive strains, demonstrating that drug-resistant parasites indeed digest hemoglobin.
利用富含57Fe的大鼠红细胞，对感染伯氏疟原虫的大鼠红细胞进行了穆斯堡尔研究，以确定表征疟色素铁的物理参数，并测试广泛使用的抗疟疾药物氯喹对这些参数的影响。发现疟色素中的铁来自细胞内寄生虫对血红蛋白的消化，呈三价、高自旋态，其穆斯堡尔参数与任何已知的含铁卟啉化合物的参数有显著差异。在用氯喹治疗前后，对感染伯氏疟原虫药物敏感株和耐药株的红细胞所获得的参数未发现差异。铁化合物由直径约30埃的微聚集体组成。在耐氯喹菌株中这些微聚集体稍大一些，在用药物处理后，在氯喹敏感菌株中其尺寸往往会增大。感染耐氯喹菌株的红细胞的穆斯堡尔光谱显示，色素铁的相对含量与氯喹敏感菌株中的相同，这表明耐药寄生虫确实会消化血红蛋白。
