Kirilov N, Petkov M, Iolov I
Vet Med Nauki. 1984;21(3):29-35.
Studied were some carbonic acids to replace asparagine in Soton's original medium in the production of PPD-tuberculins. Used were the following carbonic acids: (a) dicarboxylic saturated--oxalic, malonic, and amber acid; (b) dicarboxylic unsaturated--maleic and fumaric; and (c) dibasic oxicarbonic acids--malic and tartaric. Each of these acids participated in an equal amount as that of asparagine in replacing it in Soton's medium. Two strains were used in the experiments--AN5 and D4. Tuberculins were obtained through precipitating a filtrated material of cultures killed with trichloracetic acid. The tuberculins produced (bovine and avian PPD types) with the use of the various carbonic acids were tested on sensibilized guinea pigs and chickens parallel to the testing of standard tuberculins. The results obtained with the use of carbonic PPD-tuberculins were almost identical to those obtained with the use of standard PPD-tuberculins. The positive results make it reasonable to believe that some of the carbonic acids mentioned above may well be used in the production of PPD-tuberculins.
研究了一些碳酸用于替代索顿原始培养基中的天冬酰胺来生产结核菌素纯蛋白衍生物（PPD）。使用了以下碳酸：（a）饱和二羧酸——草酸、丙二酸和琥珀酸；（b）不饱和二羧酸——马来酸和富马酸；以及（c）二元含氧碳酸——苹果酸和酒石酸。在索顿培养基中，这些酸中的每一种在替代天冬酰胺时的用量与天冬酰胺相等。实验使用了两种菌株——AN5和D4。通过沉淀用三氯乙酸杀死的培养物的过滤材料来获得结核菌素。在致敏豚鼠和鸡身上对使用各种碳酸生产的结核菌素（牛型和禽型PPD）进行测试，同时对标准结核菌素进行测试。使用碳酸PPD结核菌素获得的结果与使用标准PPD结核菌素获得的结果几乎相同。这些阳性结果使人们有理由相信，上述某些碳酸很可能可用于生产PPD结核菌素。
