Fricker C R
Zentralbl Bakteriol Mikrobiol Hyg B. 1984 May;179(2):170-8.
Methods for the isolation of salmonellae from sewage sludge were compared. Buffered peptone water and lactose broth were compared to determine their efficiencies as preenrichment media and temperature and duration of incubation were also investigated. In addition five enrichment and five plating media were compared together with the effects of multiple plating of enrichment broths. Buffered peptone water incubated at 43 degrees C for 24 h was shown to be the pre-enrichment method of choice with enrichment in the RB 10 form of Rappaport's broth incubated at 43 degrees C for 48 h and plating at 24 and 48 h onto brilliant green agar containing sulphamandelate supplement and Hynes' modification of desoxycholate citrate agar. Of the 100 samples used in the study. 96 were found to be positive for salmonella by at least one of the procedures used. A total of 15 Salmonella serotypes were isolated. Salmonella virchow being the most common.
对从污水污泥中分离沙门氏菌的方法进行了比较。比较了缓冲蛋白胨水和乳糖肉汤，以确定它们作为预富集培养基的效率，同时还研究了培养温度和培养时间。此外，还比较了五种富集培养基和五种平板培养基，以及富集肉汤多次平板接种的效果。结果表明，43℃孵育24小时的缓冲蛋白胨水是首选的预富集方法，随后在43℃孵育48小时的RB 10型Rappaport肉汤中进行富集，并在24小时和48小时时接种到含有磺胺扁桃酸盐补充剂的亮绿琼脂和海因斯改良的脱氧胆酸盐柠檬酸盐琼脂上。在该研究使用的100个样本中，至少有一种所采用的检测程序检测出96个样本沙门氏菌呈阳性。共分离出15种沙门氏菌血清型，其中virchow沙门氏菌最为常见。
