Anselmo R J, Barrios H, Viora S, Lorent B, Eiguer T, Caffer M I, Fliess E
Universidad Nacional de Luján, Argentina.
Rev Argent Microbiol. 1989 Jul-Dec;21(3-4):127-32.
The study presented here is the first stage of a project designed to determine the presence of Salmonella serotypes in Lujan river waters, which flow beside the urban area of the homonymous city, in the province of Buenos Aires. It consisted in a comparative study of four methods for Salmonellae detection in 200 samples: Tetrathionate-brilliant green; mannitol-selenite; Rappaport 25 and modified Rappaport-Vassiliadis broth, each of them with and without pre-enrichment in buffered peptone water. The submerged gauze technique was applied to the sampling in three different spots of the river: at the town center, two km water above, and two down-stream from the city. Best results were obtained when modified Rappaport-Vassiliadis enrichment broth and brilliant green agar with 0.25% of sodium deoxycholate were used, after pre-enrichment of the samples in buffered peptone water. Salmonella were isolated from 46% of the samples studied, and 16 serotypes were identified: S. Typhimurium, S. Enteritidis, S. Infantis, S. Mbandaka, S. Israel and S. Subspecies IV 18 z36: z38:-, among them.
本文介绍的这项研究是一个项目的第一阶段，该项目旨在确定卢汉河河水中沙门氏菌血清型的存在情况，卢汉河流经布宜诺斯艾利斯省同名城市的市区。该研究包括对200个样本中沙门氏菌检测的四种方法进行比较研究：四硫磺酸盐-煌绿培养基；甘露醇-亚硒酸盐培养基；Rappaport 25培养基和改良的Rappaport-Vassiliadis肉汤培养基，每种培养基分别在有无缓冲蛋白胨水中进行预富集的情况下使用。采用浸没纱布技术在河流的三个不同地点进行采样：镇中心、镇中心上游两公里处以及镇中心下游两公里处。在缓冲蛋白胨水中对样本进行预富集后，使用改良的Rappaport-Vassiliadis富集肉汤培养基和含0.25%脱氧胆酸钠的煌绿琼脂时，获得了最佳结果。在所研究的样本中，46%分离出了沙门氏菌，鉴定出16种血清型：其中包括鼠伤寒沙门氏菌、肠炎沙门氏菌、婴儿沙门氏菌、姆班达卡沙门氏菌、以色列沙门氏菌和亚种IV 18 z36:z38:-。
