Vassiliadis P, Trichopoulos D, Pateraki E, Papaiconomou N
Zentralbl Bakteriol Orig B. 1978 Jan;166(1):81-6.
In the examination of 526 samples of minced meat for the presence of salmonellae, a preliminary pre-enrichment in buffered peptone water was made. From this medium enrichments were made, for each sample of meat, in the usual Rappaport's medium (R25), incubated at 37 degrees C, and in Muller-Kauffmanns tetrathionate broth, incubated at 43 degrees C. Moreover, an enrichment was also made in a strongly modified Rappaport's medium, containing much less malachite green (R10), incubated at 43 degrees C. The two Rappaport's media proved to be equally effective in the isolation of salmonellae and clearly more efficient, in this respect, than the Muller-Kauffmann's broth. The new Rappaport's medium (R10), incubated at 43 degrees C, has the advantage over the usuals Rappaport's and Muller-Kauffmann's enrichment media to inhibit much more considerably the growth of competing organisms than the two other enrichment media.
在对526份碎肉样本进行沙门氏菌检测时，先将样本在缓冲蛋白胨水中进行初步预富集。然后，从该培养基中对每份肉样进行富集培养，一份在常规的Rappaport培养基（R25）中于37℃培养，另一份在Muller-Kauffmann四硫磺酸盐肉汤中于43℃培养。此外，还在一种经过大幅改良、孔雀绿含量少得多的Rappaport培养基（R10）中于43℃进行富集培养。结果表明，两种Rappaport培养基在分离沙门氏菌方面同样有效，且在这方面明显比Muller-Kauffmann肉汤更高效。在43℃培养的新型Rappaport培养基（R10）相对于常规的Rappaport和Muller-Kauffmann富集培养基具有优势，它比其他两种富集培养基更能显著抑制竞争菌的生长。
