Sanders E, Moorman A F, Los J A
Anat Embryol (Berl). 1984;169(2):185-91. doi: 10.1007/BF00303148.
Immunofluorescence studies were performed on serial sections of three days embryonic chicken hearts using antibodies specific for adult atrial and ventricular myosin heavy chains respectively. The anti-ventricular myosin serum reacted with the entire myocardium showing a decreasing intensity going from the truncus arteriosus to the atrial part; however, the antiatrial myosin serum reacted weakly with the myocardium of the atrial part. Two other interesting observations were made, i) the anti-atrial myosin serum reacted with non-myocardial cells in the cardiac jelly, ii) both antisera reacted with a thin myocardial layer, extending from the ventral wall of the atrial part via the medio-dorsal wall of the atrio-ventricular canal to the dorsal wall of the ventricular part.
使用分别针对成年心房和心室肌球蛋白重链的特异性抗体，对三天龄鸡胚胎心脏的连续切片进行免疫荧光研究。抗心室肌球蛋白血清与整个心肌发生反应，从动脉干到心房部分强度逐渐降低；然而，抗心房肌球蛋白血清与心房部分的心肌反应较弱。还观察到另外两个有趣的现象：i）抗心房肌球蛋白血清与心胶质中的非心肌细胞发生反应；ii）两种抗血清均与一层薄的心肌层发生反应，该心肌层从心房部分的腹侧壁经房室管的中背壁延伸至心室部分的背壁。
