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本文引用的文献

1
Comparison of surface plasmon resonance imaging and enzyme-linked immunosorbent assay for the detection of antibodies against iridovirus in rock bream (Oplegnathus fasciatus).表面等离子体共振成像与酶联免疫吸附测定法检测条石鲷（Oplegnathus fasciatus）抗虹彩病毒抗体的比较
J Vet Diagn Invest. 2007 Jul;19(4):414-6. doi: 10.1177/104063870701900414.
2
Serodiagnostic comparison between two methods, ELISA and surface plasmon resonance for the detection of antibodies of classical swine fever.两种方法（酶联免疫吸附测定法和表面等离子体共振法）用于检测猪瘟抗体的血清学诊断比较。
J Vet Med Sci. 2006 Dec;68(12):1327-9. doi: 10.1292/jvms.68.1327.
3
Serodiagnostic comparison of enzyme-linked immunosorbent assay and surface plasmon resonance for the detection of antibody to porcine circovirus type 2.酶联免疫吸附测定法和表面等离子体共振技术用于检测猪圆环病毒2型抗体的血清学诊断比较
Can J Vet Res. 2006 Oct;70(4):263-8.
4
Serodiagnostic comparison between two methods, ELISA and surface plasmon resonance for the detection of antibody titres of Mycoplasma hyopneumoniae.两种方法（酶联免疫吸附测定法和表面等离子体共振技术）用于检测猪肺炎支原体抗体滴度的血清学诊断比较
J Vet Med B Infect Dis Vet Public Health. 2006 Mar;53(2):87-90. doi: 10.1111/j.1439-0450.2006.00917.x.
5
Comparison of culture, PCR, and different serologic tests for detection of Mycoplasma gallisepticum and Mycoplasma synoviae infections.用于检测鸡毒支原体和滑液囊支原体感染的培养、聚合酶链反应（PCR）及不同血清学检测方法的比较
Avian Dis. 2005 Jun;49(2):260-8. doi: 10.1637/7274-090804R.
6
Duplex PCR to differentiate between Mycoplasma synoviae and Mycoplasma gallisepticum on the basis of conserved species-specific sequences of their hemagglutinin genes.基于滑膜支原体和鸡毒支原体血凝素基因保守的种特异性序列，采用双重PCR法对二者进行鉴别。
J Clin Microbiol. 2005 Feb;43(2):948-58. doi: 10.1128/JCM.43.2.948-950.2005.
7
A fusion protein expression analysis using surface plasmon resonance imaging.使用表面等离子体共振成像进行融合蛋白表达分析。
Anal Biochem. 2004 Jul 15;330(2):251-6. doi: 10.1016/j.ab.2004.02.009.
8
Surface plasmon resonance imaging.表面等离子体共振成像
Anal Bioanal Chem. 2004 Jun;379(3):328-31. doi: 10.1007/s00216-004-2636-8. Epub 2004 May 5.
9
ProteoChip: a highly sensitive protein microarray prepared by a novel method of protein immobilization for application of protein-protein interaction studies.蛋白质芯片：一种通过新型蛋白质固定方法制备的高灵敏度蛋白质微阵列，用于蛋白质-蛋白质相互作用研究。
Proteomics. 2003 Dec;3(12):2289-304. doi: 10.1002/pmic.200300541.
10
Improved detection of antibodies to Mycoplasma synoviae vaccine MS-H using an autologous recombinant MSPB enzyme-linked immunosorbent assay.使用自体重组MSPB酶联免疫吸附测定法改进对滑膜支原体疫苗MS-H抗体的检测。
Avian Pathol. 2002 Dec;31(6):611-7. doi: 10.1080/0307945021000024553.

利用表面等离子体共振成像技术对鸡滑液支原体主要表面蛋白A抗体进行快速血清学诊断。

Rapid serodiagnosis with the use of surface plasmon resonance imaging for the detection of antibodies against major surface protein A of Mycoplasma synoviae in chickens.

作者信息

Oh Kiseok, Lee Semi, Seo Jayoung, Lee Dongwoo, Kim Taejung

机构信息

College of Veterinary Medicine, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju, Republic of Korea.

出版信息

Can J Vet Res. 2010 Jan;74(1):71-4.

PMID:20357963
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2801316/
Abstract

Mycoplasma synoviae, a major worldwide pathogen in poultry, causes respiratory tract infection and arthritis in chickens and turkeys. Two major surface antigens of M. synoviae are encoded by a single gene, vlhA (variably expressed lipoprotein and hemagglutinin). The gene product is cleaved post-translationally to yield the lipoprotein major surface protein (MSP) B (MSPB) and the hemagglutinin MSPA. The availability of MSPA as an antigen for serodiagnosis was studied by means of a protein chip based on surface plasmon resonance imaging (SPRi). The diagnostic potential of SPRi for measurement of levels of antibody to MSPA was compared with that of a conventional enzyme-linked immunosorbent assay (ELISA) kit. The results from SPRi, a process that took only 1 h, were similar to those from ELISA. Therefore, MSPA can be used as an antigen for serologic studies, and SPRi, a label-free and high-throughput method, may be a valuable tool in avian serodiagnostic studies.

摘要

滑液支原体是一种在全球范围内引起家禽发病的主要病原体，可导致鸡和火鸡出现呼吸道感染和关节炎。滑液支原体的两种主要表面抗原由单个基因vlhA（可变表达脂蛋白和血凝素）编码。该基因产物在翻译后被切割，产生脂蛋白主要表面蛋白（MSP）B（MSPB）和血凝素MSPA。通过基于表面等离子体共振成像（SPRi）的蛋白质芯片研究了MSPA作为血清学诊断抗原的可用性。将SPRi检测MSPA抗体水平的诊断潜力与传统酶联免疫吸附测定（ELISA）试剂盒进行了比较。仅需1小时的SPRi检测结果与ELISA检测结果相似。因此，MSPA可作为血清学研究的抗原，而SPRi作为一种无标记的高通量方法，可能是禽类血清学诊断研究中的一种有价值的工具。