体内肝脏和肾脏中眼酸的合成。
Synthesis of ophthalmic acid in liver and kidney in vivo.
作者信息
Orlowski M, Wilk S
出版信息
Biochem J. 1978 Feb 15;170(2):415-9. doi: 10.1042/bj1700415.
Abstract
The synthesis of ophthalmic acid, an analogue of glutathione, was studied in vivo in mouse liver and kidney after administration of either L-alpha-aminobutyrate or L-gamma-glutamyl-L-alpha-aminobutyrate as precursor. L-alpha-aminobutyrate accumulated to a much greater extent, and induced a much greater synthesis of ophthalmic acid in the liver than in the kidney. In contrast, L-gamma-glutamyl-L-alpha-aminobutyrate initiated a large and more rapid synthesis of ophthalmic acid in the kidney than in the liver. Experiments with L-gamma-[G(-14)C]glutamyl-L-alpha-aminobutyrate showed that, although part of the dipeptide is degraded to its constituent amino acids, a significant proportion is directly incorporated into kidney ophthalmic acid. In contrast L-gamma-glutamyl-L-alpha-aminobutyrate serves poorly as a direct precursor of liver ophthalmic acid. The present results show that kidney gamma-glutamyl tripeptide synthesis can proceed directly from an exogenous gamma-glutamyl dipeptide precursor.
摘要
在以L-α-氨基丁酸酯或L-γ-谷氨酰-L-α-氨基丁酸酯作为前体给药后，在小鼠肝脏和肾脏中对谷胱甘肽类似物眼酸的合成进行了体内研究。L-α-氨基丁酸酯在肝脏中的积累程度要大得多，并且诱导肝脏中眼酸的合成比在肾脏中多得多。相比之下，L-γ-谷氨酰-L-α-氨基丁酸酯在肾脏中引发的眼酸合成比在肝脏中更大且更快。用L-γ-[G(-14)C]谷氨酰-L-α-氨基丁酸酯进行的实验表明，虽然部分二肽会降解为其组成氨基酸，但相当一部分会直接掺入肾脏眼酸中。相比之下，L-γ-谷氨酰-L-α-氨基丁酸酯作为肝脏眼酸的直接前体效果不佳。目前的结果表明，肾脏γ-谷氨酰三肽合成可以直接从外源性γ-谷氨酰二肽前体进行。
相似文献
1
Synthesis of ophthalmic acid in liver and kidney in vivo.体内肝脏和肾脏中眼酸的合成。
Biochem J. 1978 Feb 15;170(2):415-9. doi: 10.1042/bj1700415.
2
Metabolism of gamma-glutamyl amino acids and peptides in mouse liver and kidney in vivo.小鼠肝脏和肾脏中γ-谷氨酰氨基酸和肽的体内代谢
Eur J Biochem. 1976 Dec 11;71(2):549-55. doi: 10.1111/j.1432-1033.1976.tb11144.x.
3
Intermediates of the gamma-glutamyl cycle in mouse tissues. Influence of administration of amino acids on pyrrolidone carboxylate and gamma-glutamyl amino acids.小鼠组织中γ-谷氨酰循环的中间产物。氨基酸给药对吡咯烷酮羧酸盐和γ-谷氨酰氨基酸的影响。
Eur J Biochem. 1975 May 6;53(2):581-90. doi: 10.1111/j.1432-1033.1975.tb04101.x.
4
L-gamma-(Threo-beta-methyl)glutamyl-L-alpha-aminobutyrate, a selective substrate of alpha-glutamyl cyclotransferase.L-γ-（苏式-β-甲基）谷氨酰-L-α-氨基丁酸，α-谷氨酰环化转移酶的一种选择性底物。
J Biol Chem. 1980 Nov 25;255(22):10787-92.
5
Glutathione turnover in the kidney; considerations relating to the gamma-glutamyl cycle and the transport of amino acids.肾脏中的谷胱甘肽周转；与γ-谷氨酰循环及氨基酸转运相关的考量
Proc Natl Acad Sci U S A. 1974 Aug;71(8):2969-72. doi: 10.1073/pnas.71.8.2969.
6
[Does a modified gamma-glutamyl cycle exist in human erythrocytes (author's transl)].人类红细胞中是否存在改良的γ-谷氨酰循环（作者译）
Hoppe Seylers Z Physiol Chem. 1976 Nov;357(11):1459-63.
7
Kidney as a site of uptake and metabolism of gamma-glutamyl compounds.肾脏作为γ-谷氨酰化合物的摄取和代谢部位。
Curr Probl Clin Biochem. 1977;8:66-72.
8
Transport of gamma-glutamyl amino acids: role of glutathione and gamma-glutamyl transpeptidase.γ-谷氨酰氨基酸的转运：谷胱甘肽和γ-谷氨酰转肽酶的作用
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6319-22. doi: 10.1073/pnas.76.12.6319.
9
The fate of extracellular glutathione in the rat.大鼠体内细胞外谷胱甘肽的命运
Biochim Biophys Acta. 1978 Mar 20;539(3):324-37. doi: 10.1016/0304-4165(78)90037-5.
10
Ophthalmic acid accumulation in an Escherichia coli mutant lacking the conserved pyridoxal 5'-phosphate-binding protein YggS.在缺乏保守的磷酸吡哆醛结合蛋白YggS的大肠杆菌突变体中眼酸的积累。
J Biosci Bioeng. 2016 Dec;122(6):689-693. doi: 10.1016/j.jbiosc.2016.06.010. Epub 2016 Jul 12.
引用本文的文献
1
Ophthalmate is a new regulator of motor functions via CaSR: implications for movement disorders.眼氨酸通过钙敏感受体调节运动功能：对运动障碍的影响。
Brain. 2024 Oct 3;147(10):3379-3394. doi: 10.1093/brain/awae097.
2
New K50R mutant mouse models reveal impaired hypusination of eif5a2 with alterations in cell metabolite landscape.新型 K50R 突变小鼠模型揭示 eif5a2 的低亚氨酸化受损，伴有细胞代谢物图谱改变。
Biol Open. 2023 Mar 15;12(3). doi: 10.1242/bio.059647. Epub 2023 Mar 21.
3
Frailty in Aging and the Search for the Optimal Biomarker: A Review.衰老中的衰弱与最佳生物标志物的探寻：综述
Biomedicines. 2022 Jun 16;10(6):1426. doi: 10.3390/biomedicines10061426.
4
Metabolic impact of pathogenic variants in the mitochondrial glutamyl-tRNA synthetase EARS2.线粒体谷氨酰-tRNA 合成酶 EARS2 中致病性变异对代谢的影响。
J Inherit Metab Dis. 2021 Jul;44(4):949-960. doi: 10.1002/jimd.12387. Epub 2021 Apr 27.
5
Diverse metabolic reactions activated during 58-hr fasting are revealed by non-targeted metabolomic analysis of human blood.非靶向代谢组学分析揭示了人类血液在 58 小时禁食期间激活的多种代谢反应。
Sci Rep. 2019 Jan 29;9(1):854. doi: 10.1038/s41598-018-36674-9.
6
Metabolic adaptation of short-living growth hormone transgenic mice to methionine restriction and supplementation.短期生长激素转基因小鼠对蛋氨酸限制和补充的代谢适应。
Ann N Y Acad Sci. 2018 Apr;1418(1):118-136. doi: 10.1111/nyas.13687.
7
Inability to maintain GSH pool in G6PD-deficient red cells causes futile AMPK activation and irreversible metabolic disturbance.葡萄糖-6-磷酸脱氢酶（G6PD）缺乏的红细胞无法维持谷胱甘肽（GSH）池，导致无效的腺苷酸活化蛋白激酶（AMPK）激活和不可逆的代谢紊乱。
Antioxid Redox Signal. 2015 Mar 20;22(9):744-59. doi: 10.1089/ars.2014.6142. Epub 2015 Feb 10.
8
A distinct metabolic signature of human colorectal cancer with prognostic potential.具有预后潜力的人类结直肠癌独特代谢特征。
Clin Cancer Res. 2014 Apr 15;20(8):2136-46. doi: 10.1158/1078-0432.CCR-13-1939. Epub 2014 Feb 13.
9
Dynamics of glutathione and ophthalmate traced with 2H-enriched body water in rats and humans.用富含氘的身体水分追踪大鼠和人类中谷胱甘肽和眼氨酸的动态变化。
Am J Physiol Endocrinol Metab. 2009 Jul;297(1):E260-9. doi: 10.1152/ajpendo.00080.2009. Epub 2009 Apr 28.
10
Peptidases in the kidney and urine of rats after castration.去势大鼠肾脏和尿液中的肽酶
Histochemistry. 1982;74(1):63-84. doi: 10.1007/BF00495053.
本文引用的文献
1
Synthesis of peptides in enzymic reactions involving glutathione.涉及谷胱甘肽的酶促反应中肽的合成。
Nature. 1950 Aug 19;166(4216):288-92. doi: 10.1038/166288a0.
2
Enzymic transpeptidation reactions involving gamma-glutamyl peptides and alpha-amino-acyl peptides.涉及γ-谷氨酰肽和α-氨基酰肽的酶促转肽反应。
Biochem J. 1952 Apr;51(1):25-35. doi: 10.1042/bj0510025.
3
Acidic peptides of the lens. 6. Metabolism of gamma-glutamyl peptides in subcellular fractions of rabbit liver.晶状体的酸性肽。6. 兔肝脏亚细胞组分中γ-谷氨酰肽的代谢
Biochem J. 1961 Apr;79(1):118-28. doi: 10.1042/bj0790118.
4
Acidic peptides of the lens. 4. The biosynthesis of ophthalmic acid.晶状体的酸性肽。4. 眼酸的生物合成。
Biochem J. 1958 Aug;69(4):649-55. doi: 10.1042/bj0690649.
5
Acidic peptides of the lens.晶状体的酸性肽
Biochem J. 1956 Dec;64(4):715-26. doi: 10.1042/bj0640715.
6
Enzymic formation of pyrrolidone carboxylic acid from gamma-glutamyl peptides.由γ-谷氨酰肽通过酶促反应形成吡咯烷酮羧酸。
Nature. 1956 Feb 25;177(4504):377-8. doi: 10.1038/177377a0.
7
Formation and utilization of gamma-glutamylcysteine in glutathione synthesis.
J Biol Chem. 1952 Nov;199(1):407-14.
8
The estimation of glutathione in rat tissues. A comparison of a new spectrophotometric method with the glyoxalase method.大鼠组织中谷胱甘肽的测定。一种新的分光光度法与乙二醛酶法的比较。
Biochem J. 1964 Nov;93(2):232-6. doi: 10.1042/bj0930232.
9
Isolation of highly purified gamma-glutamylcysteine synthetase from rat kidney.从大鼠肾脏中分离高纯度的γ-谷氨酰半胱氨酸合成酶。
Biochemistry. 1971 Feb 2;10(3):372-80. doi: 10.1021/bi00779a003.
10
Enzymatic conversion of 5-oxo-L-proline (L-pyrrolidone carboxylate) to L-glutamate coupled with cleavage of adenosine triphosphate to adenosine diphosphate, a reaction in the -glutamyl cycle.5-氧代-L-脯氨酸（L-吡咯烷酮羧酸盐）酶促转化为L-谷氨酸，并伴有三磷酸腺苷裂解为二磷酸腺苷，这是γ-谷氨酰循环中的一个反应。
Proc Natl Acad Sci U S A. 1971 Dec;68(12):2982-5. doi: 10.1073/pnas.68.12.2982.
Zotero 插件