Expression of cloned cDNA for a major surface antigen of Plasmodium falciparum merozoites.
作者信息
Odink K G, Lockyer M J, Nicholls S C, Hillman Y, Freeman R R, Holder A A
出版信息
FEBS Lett. 1984 Jul 23;173(1):108-12. doi: 10.1016/0014-5793(84)81027-3.
PMID:6378659
Abstract
A cDNA library of P. falciparum was constructed. Using size-selected mRNA as a probe several clones were isolated which hybridized to mRNAs larger than 5 kilobases (kb). The cDNA insert of pFC 17, which hybridizes to 5.6-kb mRNA was expressed by fusion to anthranilate synthetase I in a plasmid expression vector. The expressed fusion protein was shown to contain epitopes of a 195-kDa protein which is the precursor to 3 major surface antigens of P. falciparum merozoites.
摘要
Zotero 插件