恶性疟原虫一种输出抗原的基因在大肠杆菌中被克隆并表达。
The gene for an exported antigen of the malaria parasite Plasmodium falciparum cloned and expressed in Escherichia coli.
作者信息
Hope I A, Mackay M, Hyde J E, Goman M, Scaife J
出版信息
Nucleic Acids Res. 1985 Jan 25;13(2):369-79. doi: 10.1093/nar/13.2.369.
An exported protein of the erythrocytic stages of the malaria parasite, Plasmodium falciparum, has epitope(s) in common with the surface of the sporozoite stage (1). Two cDNA clones encoding this protein, Ag5.1, have now been isolated and expressed in Escherichia coli. The coding sequence contains a region with strong homology to that of the circumsporozoite protein of P. falciparum. Other features of the sequence can be explained in terms of the observed behaviour of the protein in the parasite life cycle. The Ag5.1 can now be synthesised in bacteria in sufficient amounts to analyse the immune response to this protein.
恶性疟原虫红细胞阶段的一种输出蛋白在表位上与子孢子阶段的表面具有共同之处(1)。现在已分离出编码该蛋白Ag5.1的两个cDNA克隆，并在大肠杆菌中进行了表达。编码序列包含一个与恶性疟原虫环子孢子蛋白具有高度同源性的区域。该序列的其他特征可以根据在寄生虫生命周期中观察到的该蛋白行为来解释。现在可以在细菌中大量合成Ag5.1，以分析对该蛋白的免疫反应。
Zotero 插件