Burrows P M, Scott S W, Barnett O W, McLaughlin M R
J Virol Methods. 1984 May;8(3):207-16. doi: 10.1016/0166-0934(84)90015-6.
Precise use of enzyme-linked immunosorbent assay (ELISA) as a quantitative technique depends on repeatability of color development and its measurement. Variation in measured response among wells, within and among microtiter plates, often precludes such precision. For example, plates with all wells treated uniformly exhibited unacceptable optical density differences in excess of 0.35 and 0.25 O.D. U among row and column averages, respectively. Arrangement of samples on plates according to classical experimental designs, with compact blocking features and two-dimensional control over spatial patterns, provides a possible remedy. Analysis of variations over uniformly treated plates demonstrated the potential for increased precision when such designs are used instead of random arrangements. Retrospective analysis of more than 100 tests performed with various experimental designs confirmed that this potential was realized when using Youden square and lattice square designs. Several designs appropriate for microtiter plates are presented and their conduct described.
将酶联免疫吸附测定（ELISA）精确用作定量技术取决于显色及其测量的可重复性。微孔板内各孔之间以及不同微孔板之间测量响应的差异，常常妨碍这种精确性。例如，所有孔均一处理的板在行平均值和列平均值之间分别表现出超过0.35和0.25光密度单位的不可接受的光密度差异。根据经典实验设计在板上排列样品，具有紧凑的区组特征和对空间模式的二维控制，提供了一种可能的补救方法。对均一处理板上的变异分析表明，使用此类设计而非随机排列时，有提高精确性的潜力。对采用各种实验设计进行的100多次测试的回顾性分析证实，使用尤登方和格子方设计时，这种潜力得以实现。本文介绍了几种适用于微孔板的设计并描述了其操作方法。
