一种通过低倍透射电子显微镜定位膜结合单克隆抗体的生物素-抗生物素蛋白技术。
A biotin-avidin technique for the localisation of membrane-bound monoclonal antibodies by low power transmission electron microscopy.
作者信息
Volsen S G
出版信息
J Immunol Methods. 1984 Aug 3;72(1):119-26. doi: 10.1016/0022-1759(84)90439-3.
PMID:6379052
Abstract
A biotin-avidin horseradish peroxidase system is described for the localisation of membrane-bound monoclonal antibodies by low power transmission electron microscopy. An indirect bridge technique was used, with a biotin-labelled goat anti-mouse immunoglobulin antibody and horseradish peroxidase-conjugated avidin D. The technique provided preparations of high morphological and ultrastructural quality which facilitated accurate cellular identification. Positive immunostaining was specific and precisely defined.
摘要
本文描述了一种生物素-抗生物素蛋白辣根过氧化物酶系统，用于通过低倍透射电子显微镜定位膜结合单克隆抗体。采用间接桥接技术，使用生物素标记的山羊抗小鼠免疫球蛋白抗体和辣根过氧化物酶偶联的抗生物素蛋白D。该技术提供了形态和超微结构质量高的标本，便于准确进行细胞识别。阳性免疫染色具有特异性且定义精确。
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