Kelly D C, Lescott T
J Gen Virol. 1984 Jul;65 ( Pt 7):1183-91. doi: 10.1099/0022-1317-65-7-1183.
A number of polypeptides synthesized specifically in Trichoplusia ni multiple nucleocapsid nuclear polyhedrosis virus (T. ni MNPV)-infected Spodoptera frugiperda cells are phosphorylated both early and late in infection. Certain non-structural proteins and the major basic internal protein are the main phosphoproteins detected in infected cells. The polyhedron protein was not phosphorylated. Many cell proteins continue to be phosphorylated throughout infection. Pulse-chase experiments have shown that some polypeptides are stably phosphorylated whereas other polypeptides (including the major basic protein) have phosphates which cycle on and off. One polypeptide was substantially labelled only after a chase with unlabelled orthophosphate. Fractionation of cells into nucleus and cytoplasm showed that polypeptides located in both the cytoplasm and nucleus were phosphorylated.
在被粉纹夜蛾多核衣壳核型多角体病毒（T. ni MNPV）感染的草地贪夜蛾细胞中，特异性合成的多种多肽在感染的早期和晚期都会发生磷酸化。某些非结构蛋白和主要的碱性内部蛋白是在感染细胞中检测到的主要磷蛋白。多角体蛋白未被磷酸化。在整个感染过程中，许多细胞蛋白持续发生磷酸化。脉冲追踪实验表明，一些多肽被稳定磷酸化，而其他多肽（包括主要的碱性蛋白）的磷酸盐会循环地结合和脱离。一种多肽仅在用未标记的正磷酸盐追踪后才被大量标记。将细胞分离为细胞核和细胞质后发现，位于细胞质和细胞核中的多肽都会发生磷酸化。
