利用微型Gal质粒对接合性R质粒进行快速不相容性分组
The use of mini-Gal plasmids for rapid incompatibility grouping of conjugative R plasmids.
作者信息
Davey R B, Bird P I, Nikoletti S M, Praszkier J, Pittard J
出版信息
Plasmid. 1984 May;11(3):234-42. doi: 10.1016/0147-619x(84)90029-5.
PMID:6379710
Abstract
The galactose operon of Escherichia coli K-12 has been used as a phenotypic marker for miniplasmids derived in vitro from R plasmids representing six incompatibility groups. This has enabled the development of a rapid incompatibility typing scheme in which the miniplasmids are used as incompatibility exemplars, their presence in strains being monitored on galactose fermentation indicator media.
摘要
大肠杆菌K-12的半乳糖操纵子已被用作从代表六个不相容群的R质粒体外衍生的微型质粒的表型标记。这使得能够开发一种快速的不相容性分型方案，其中微型质粒用作不相容性范例，通过在半乳糖发酵指示培养基上监测菌株中微型质粒的存在情况来进行分型。
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