Crockard A D, MacFarlane E, Andrews C, Bridges J M, Catovsky D
Am J Clin Pathol. 1984 Sep;82(3):294-9. doi: 10.1093/ajcp/82.3.294.
Dipeptidylaminopeptidase IV (DAP IV) cytochemical reactivity was investigated in monoclonal antibody defined T-lymphocyte subpopulations from normal blood and in cells from a series of T-cell leukemias of defined immunologic phenotype. A combined monoclonal antibody/immunocolloidal gold technic, which enabled simultaneous visualisation of immunogold label and DAP IV reactivity, was used to study enzyme reactivity in normal T-cell subpopulations. Single or several discrete granules of DAP IV reaction product were observed in 72% of OKT3+ and OKT4+ cells, whereas a significantly (P less than 0.01) lower percentage of OKT8+ cells (41%) displayed positivity; B-cells were invariably DAP IV negative. In the T-cell leukemias, DAP IV reactivity was strongest in T-lymphoblastic lymphoma and T-prolymphocytic leukemia cells. In contrast, DAP IV activity was absent or expressed in a minority of cells of the more immature T-acute lymphoblastic leukemia. The enzyme reaction also was negative in lymphocytes from other mature T-cell leukemias: T-chronic lymphocytic leukemia (OKT8+), adult T-cell lymphoma-leukemia, and Sezary syndrome (both OKT4+). DAP IV expression did not parallel that of acid phosphatase or alpha-naphthyl acetate esterase in leukemic T-lymphoid cells except in T-lymphoblastic lymphoma and T-prolymphocytic leukemia, where a strong reaction with the three hydrolytic enzymes was observed.
研究了二肽基氨基肽酶IV（DAP IV）在正常血液中由单克隆抗体定义的T淋巴细胞亚群以及一系列具有明确免疫表型的T细胞白血病细胞中的细胞化学反应性。采用一种结合单克隆抗体/免疫胶体金技术，该技术能够同时可视化免疫金标记和DAP IV反应性，用于研究正常T细胞亚群中的酶反应性。在72%的OKT3+和OKT4+细胞中观察到单个或几个离散的DAP IV反应产物颗粒，而OKT8+细胞的阳性百分比显著较低（P小于0.01），为41%；B细胞始终为DAP IV阴性。在T细胞白血病中，DAP IV反应性在T淋巴母细胞淋巴瘤和T原淋巴细胞白血病细胞中最强。相比之下，在更不成熟的T急性淋巴细胞白血病的少数细胞中不存在或仅少量表达DAP IV活性。在其他成熟T细胞白血病的淋巴细胞中酶反应也为阴性：T慢性淋巴细胞白血病（OKT8+）、成人T细胞淋巴瘤白血病和Sezary综合征（均为OKT4+）。除了在T淋巴母细胞淋巴瘤和T原淋巴细胞白血病中观察到与三种水解酶的强反应外，白血病T淋巴细胞中的DAP IV表达与酸性磷酸酶或α-萘乙酸酯酶的表达不平行。
